Alternative names

Technical specifications

• Antigen: CD158b2 (KIR2DL3)
• Clone: REAL145
• Isotype control: REAL Control
• Alternative names of antigen: KIR2DL3, Killer cell immunoglobulin-like receptor 2DL3
• Distribution of antigen: T cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: FITC, APC

• Figure 1

  Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD158b2 (KIR2DL3)-APC (B) or with the corresponding REAL Control (A). REAlease CD158b2 (KIR2DL3)-APC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD158b2 (KIR2DL3)-APC (D). Cells were labeled with CD56 antibodies in addition and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Figure 2

  Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD158b2 (KIR2DL3) Complexes or with the corresponding REAL Control (left images) as well as with CD56 antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

• Selected references

  1. Keaney, L. et al. (2004) Investigation of killer cell immunoglobulin-like receptor gene diversity III. KIR2DL3. Tissue Antigens 64(2): 188-194
  2. Gendzekhadze, K. et al. (2009) Co-evolution of KIR2DL3 with HLA-C in a human population retaining minimal essential diversity of KIR and HLA class I ligands. Proc. Natl. Acad. Sci. U.S.A. 106: 18692-18697