Alternative names

Technical specifications

• Antigen: TIM-3
• Clone: REAL168
• Isotype control: REAL Control
• Alternative names of antigen: Tim3
• Distribution of antigen: T cells, macrophages
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: APC

• Figure 1

  Splenocytes from C57BL6 mice were stained with REAlease Anti-TIM-3-APC (B) or with the corresponding REAL Control (A). REAlease Anti-TIM-3-APC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease Anti-TIM-3-APC (D). Cells were labeled with CD11b antibodies in addition and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Selected references

  1. Monney, L. et al. (2002) Tʜ1-specific cell surface protein Tim-3 regulates macrophage activation and severity of an autoimmune disease. Nature 415: 536-541
  2. van de Weyer, P. S. et al. (2006) A highly conserved tyrosine of Tim-3 is phosphorylated upon stimulation by its ligand galectin-9. Biochem. Biophys. Res. Commun. 351(2): 571-576