Alternative names

Technical specifications

• Antigen: Ly-49H
• Clone: REAL196
• Isotype control: REAL Control
• Alternative names of antigen: Klra8, Cmv-1, Cmv1
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: FITC

• Figure 1

  Splenocytes from C57BL6 mice were stained with REAlease Anti-Ly-49H-FITC (B) or with the corresponding REAL Control (A). REAlease Anti-Ly-49H-FITC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease Anti-Ly-49H-FITC (D). Cells were labeled with CD49b antibodies in addition and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Selected references

  1. Smith, K. M. et al. (1989) Ly-49D and Ly-49H associate with mouse DAP12 and form activating receptors. J. Immunol. 161: 7-10
  2. Seung-Hwan, L. et al. (2003) Transgenic expression of the activating natural killer receptor Ly49H confers resistance to cytomegalovirus in genetically susceptible mice. J. Exp. Med. 197(4): 515-526
  3. Seung-Hwan, L. et al. (2009) Activating receptors promote NK cell expansion for maintenance, IL10 production, and CD8 T cell regulation during viral infection. J. Exp. Med. 206: 2235-2251