Alternative names

Technical specifications

• Antigen: CD38
• Clone: REAL181
• Isotype control: REAL Control
• Alternative names of antigen: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, ADPRC 1, I-19, NIM-R5, T10
• Distribution of antigen: B cells, T cells, monocytes, macrophages
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: PE, VioGreen, FITC

• Figure 1

  Splenocytes from mice were stained with REAlease CD38-FITC (B) or with the corresponding REAL Control (A). REAlease CD38-FITC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD38-FITC (D). Cells were analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Figure 2

  Splenocytes from mice were stained with REAlease CD38 Complexes or with the corresponding REAL Control (left images) and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

• Selected references

  1. Howard, M. et al. (1993) Formation and hydrolysis of cyclic ADP-ribose catalyzed by lymphocyte antigen CD38. Science 262(5136): 1056-1059