Alternative names

Technical specifications

• Antigen: CD45R (B220)
• Clone: REAL132
• Isotype control: REAL Control
• Alternative names of antigen: Ptprc, B220, CD45, loc, L-CA, Ly-5, Lyt-4, T200
• Distribution of antigen: B cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: PE, APC, VioGreen, FITC

• Figure 1

  Splenocytes from mice were stained with REAlease CD45R (B220)-FITC (B) or with the corresponding REAL Control (A). REAlease CD45R (B220)-FITC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD45R (B220)-FITC (D). Cells were labeled with CD19 antibodies in addition and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Figure 2

  Splenocytes from mice were stained with REAlease CD45R (B220) Complexes or with the corresponding REAL Control (left images) as well as with CD19 antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

• Selected references

  1. Coffman, R. L. (1982) Surface antigen expression and immunoglobulin gene rearrangement during mouse pre-B cell development. Immunol. Rev. 69: 5-23
  2. Katavic, V. et al. (2003) The surface antigen CD45R identifies a population of estrogen-regulated murine marrow cells that contain osteoclast precursors. Bone 32(6): 581-590