YILIMART - CD62L-FITC, human, REAL163, 100 tests

Clone REAL163 is an antibody fragment derived from the full CD62L antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.

Clone REAL163 recognizes the human CD62L antigen, a 74 kDa single-pass type I membrane protein which is also known as L-selectin, LECAM-1, or LAM-1. CD62L is a member of the selectin family of cell surface molecules and binds a series of glycoproteins including CD34, GlyCAM-1, and MAdCAM-1. Most hematopoietic cells express CD62L, including peripheral blood B cells, T cells, monocytes, granulocytes, thymocytes, and some myeloid cells from bone marrow. Expression on resting naive, central memory, and some effector memory T cells regulates their migration via endothelial venules to peripheral lymph nodes and Peyer’s patches. The CD62L antigen also contributes to the recruitment of leukocytes from the blood to areas of inflammation. Disruption of CD62L expression has detrimental effects on T cell migration and immune responses. Antigenic activation in the lymph node leads first to rapid CD62L shedding by protease cleavage and then to transcriptional suppression.

Always use fresh material for immunofluorescent staining of CD62L

⁺

cells. For optimal results, the cells should not be older than 8–12 hours. Keep cells continuously cold. CD62L-expression may be rapidly lost due to shedding.

The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

L-selectin, LAM-1, LECAM-1, Leu-8, TQ1, gp90-MEL, Lnhr, LSEL, Lyam1, PLNHR

Technical specifications

Antigen: CD62L
Clone: REAL163
Isotype control: REAL Control
Alternative names of antigen: L-selectin, LAM-1, LECAM-1, Leu-8, TQ1, gp90-MEL, Lnhr, LSEL, Lyam1, PLNHR
Cross-reactivity:

cynomolgus monkey (
Macaca fascicularis
)

,

rhesus monkey (
Macaca mulatta
)

, baboon
Distribution of antigen: B cells, T cells, monocytes, granulocytes, thymocytes, myeloid cells
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: FITC, PE, APC, VioGreen

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD62L-FITC (B) or with the corresponding REAL Control (A). REAlease CD62L-FITC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD62L-FITC (D). Cells were labeled with CD4 antibodies in addition and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

A:	B:

C:	D:

Figure 2

Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD62L Complexes or with the corresponding REAL Control (left images) as well as with CD4 antibodies and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

Selected references
1. Ivetic, A. (2013) Signals regulating L-selectin-dependent leucocyte adhesion and transmigration. Int. J. Biochem. Cell Biol. 45(3): 550-555
2. Telen, M. J. (2014) Cellular adhesion and the endothelium: E-selectin, L-selectin, and pan-selectin inhibitors. Hematol. Oncol. Clin. North Am. 28(2): 341-354
3. McEver, R. P. (2015) Selectins: initiators of leucocyte adhesion and signalling at the vascular wall. Cardiovasc. Res. 107(3): 331-339

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CD62L-FITC, human, REAL163, 100 tests

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Alternative names

Technical specifications

Figure 1

Figure 2

Selected references

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