YILIMART - CD146-APC, human, 541-10B2, 30 tests

Clone 541-10B2 recognizes the human CD146 antigen, also known as MUC18, MCAM, Mel‑CAM, or S-endo. CD146 is a transmembrane glycoprotein belonging to the immunoglobulin superfamily and contains five extracellular immunoglobulin (Ig)–like domains. CD146 possesses a limited tissue expression distribution, including endothelial cells, pericytes, smooth muscle cells, follicular dendritic cells, melanoma cells, and a sub-population of activated T lymphocytes. CD146 is also expressed on marrow stromal cells (MSCs).

CD146 functions as a Ca

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-independent cell adhesion molecule that is involved in heterophilic cell-cell interactions, and may be involved in the extravasion and/or homing of activated T cells. CD146 expression in melanoma is also directly associated with tumor growth and metastasis.

Alternative names

MCAM, MUC18, Endo-CAM, Mel-CAM, S-endo

Technical specifications

Antigen: CD146
Clone: 541-10B2
Isotype: mouse IgG1κ
Isotype control: Mouse IgG1 – isotype control antibodies
Alternative names of antigen: MCAM, MUC18, Endo-CAM, Mel-CAM, S-endo
Entrez Gene ID: 4162
Molecular mass of antigen [kDa]: 69
Distribution of antigen: bone marrow, dendritic cells, endothelial cells, epithelial cells, fibroblasts, mesenchymal stem cells, ES and iPS cells, smooth muscle, T cells
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: APC, PE, VioBlue

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were spiked with human umbilical vein endothelial cells (HUVECs), stained with CD146 antibodies as well as with CD45 antibodies, and analyzed by flow cytometry using the MACSQuant

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Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

Selected references
1. Pickl, W. F. et al. (1997) MUC18/MCAM (CD146), an activation antigen of human T lymphocytes. J. Immunol. 158: 2107-2115
2. Bardin, N. et al. (2001) Identification of CD146 as a component of the endothelial junction involved in the control of cell-cell cohesion. Blood 98: 3677-3684
3. Yan, X. et al. (2003) A novel anti-CD146 monoclonal antibody, AA98, inhibits angiogenesis and tumor growth. Blood 102: 184-191
4. Xie, S. et al. (1997) Expression of MCAM/MUC18 by human melanoma cell leads to increased tumor growth and metastasis. Cancer Res. 57: 2295-2303
5. Mueller, I. et al. (2006) Animal serum-free culture conditions for isolation and expansion of multipotent mesenchymal stromal cells from human BM. Cytotherapy 8: 437-444
6. Shih, J. M. (1999) The role of CD146 (Mel-CAM) in biology and pathology. J. Pathol. 189(1): 4-11
7. Satyamoorthy, K. et al. (2001) Mel-CAM-specific genetic suppressor elements inhibit melanoma growth and invasion through loss of gap junctional communication. Oncogene 20: 4676-4684

CD146-APC, human, 541-10B2, 30 tests

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Figure 1

Selected references

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