Alternative names

Technical specifications

• Antigen: CD133/2 (293C3)
• Clone: 293C3
• Isotype: mouse IgG2bκ
• Isotype control: Mouse IgG2b – isotype control antibodies
• Alternative names of antigen: PROM1, AC133, CORD12, MCDR2, MSTP061, PROML1, RP41, STGD4
• Entrez Gene ID: 8842
• Molecular mass of antigen [kDa]: 117
• Distribution of antigen: brain, endothelial cells, epithelial cells, heart, hematopoietic stem cells, kidney, liver, lung, pancreas, placenta, ES and iPS cells, red blood cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: pure, PE-Vio615

• Figure 1

  Human peripheral blood mononuclear cells (PBMCs) were stained with CD133/2 (293C3) antibodies as well as with CD34 and CD45 antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. For all other conjugates the FcR Blocking Reagent has been used to avoid Fc receptor–mediated antibody labeling. A pre-gate of CD45 + cells was used. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

• Selected references

  1. Bühring, H. J. et al. (1991) The product of the proto-oncogene c-kit (P145c-kit) is a human bone marrow surface antigen of hematopoietic precursor cells which is expressed on a subset of acute non-lymphoblastic cells. Leukemia 5: 854-860
  2. Miraglia, S. et al. (1997) A novel five-transmembrane hematopoietic stem cell antigen: isolation, characterization, and molecular cloning. Blood 90(12): 5013-5021
  3. Yin, A. H. et al. (1997) AC133, a novel marker for human hematopoietic stem and progenitor cells. Blood 90: 5002-5012
  4. Weigmann, A. et al. (1997) Prominin, a novel microvilli-specific polytopic membrane proteine of the apical surface of epithelial cells, is targeted to plasmalemmal protrusions of non-epithelial cells. Proc. Natl. Acad. Sci. U.S.A. 94: 12425-12430
  5. de Wynter, E. A. et al. (1998)
     CD34

     ⁺

     AC133

     ⁺

     cells isolated from cord blood are highly enriched in long-term culture-initiating cells, NOD/SCID-repopulating cells and dendritic cell progenitors.
     Stem Cells 16: 387-396
  6. Bühring, H. J. et al. (1999) Expression of novel surface antigens on early hematopoietic cells. Ann. N. Y. Acad. Sci. 872: 25-39
  7. Gallacher, L. et al. (2000)
     Isolation and characterization of human CD34

     ^–

     Lin

     ^–

     and CD34

     ⁺

     Lin

     ^–

     hematopoietic stem cells using cell surface markers AC133 and CD7.
     Blood 95(ARVO Annual Meeting Abstract): 2813-2820
  8. Peichev, M. et al. (2000)
     Expression of VEGFR-2 and AC133 by circulating human CD34

     ⁺

     cells identifies a population of functional endothelial precursors.
     Blood 95: 952-958
  9. Uchida, N. et al. (2000) Direct isolation of human central nervous system stem cells. Proc. Natl. Acad. Sci. U.S.A. 97: 14720-14725
  10. Richardson, G. et al. (2004) CD133, a novel marker for human prostatic epithelial stem cells. J. Cell. Sci. 117: 3539-3545
  11. Thill, M. et al. (2004)
      Identification of a population of CD133

      ⁺

      precursor cells in the stroma of human cornea.
      Invest. Ophthalmol. Vis. Sci. 45: 3519
  12. Bussolati, B. et al. (2005) Isolation of renal progenitor cells from adult human kidney. Am. J. Pathol. 166: 545-555
  13. Pfenninger, C. V. et al. (2007) CD133 is not present on neurogenic astrocytes in the adult subventricular zone, but on embryonic neural stem cells, ependymal cells, and glioblastoma cells. Cancer Res. 67(12): 5727-5736
  14. Hawley, R. G. et al. (2006) Hematopoietic stem cells. Meth. Enzymol. 419: 149-179
  15. Cox, J. L. et al. (2012) Elevating SOX2 levels deleteriously affects the growth of medulloblastoma and glioblastoma cells. PLoS One 7(8): e44087
  16. Balasubramanian, P. et al. (2013)
      AQP9 expression in glioblastoma multiforme tumors is limited to a small population of astrocytic cells and CD15

      ⁺

      /CalB

      ⁺

      leukocytes.
      PLoS One 8(9): e75764
  17. Walton, R. M. et al. (2013)
      Postnatal neural precursor cell regions in the rostral subventricular zone, hippocampal subgranular zone and cerebellum of the dog (

      Canis lupus familiaris

      ).
      Histochem. Cell Biol. 139(3): 415-429
  18. Donnenberg, A. D. et al. (2013) KIT (CD117) expression in a subset of non-small cell lung carcinoma (NSCLC) patients. PLoS One 7(12): e52885
  19. Kurian, L. et al. (2013) Conversion of human fibroblasts to angioblast-like progenitor cells. Nat. Methods 10(1): 77-83
  20. Kang, T. W. et al. (2014) Growth arrest and forced differentiation of human primary glioblastoma multiforme by a novel small molecule. Sci. Rep. 4: 5546
  21. Golden, J. E. et al. (2012) Human umbilical cord blood cells alter blood and spleen cell populations after stroke. Transl Stroke Res 3(4): 491-499
  22. Wang, L. et al. (2013) Enrichment of prostate cancer stem-like cells from human prostate cancer cell lines by culture in serum-free medium and chemoradiotherapy. Int. J. Biol. Sci. 9(5): 472-479

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