YILIMART - CD133/1 (W6B3C1) pure, human

CD133 is found on multipotent progenitor cells, including immature hematopoietic stem and progenitor cells. in human fetal liver, bone marrow, cord blood, and peripheral blood. CD133 has also been found to be expressed on circulating endothelial progenitor cells and other tissue cells such as brain

^1,2

, prostatic tissue

³

, muscle

⁴

, or stroma of the cornea

⁵

, cancer stem cells from tumor tissues, as well as ES and iPS cell-derived cells.

Alternative names

PROM1, AC133, CORD12, MCDR2, MSTP061, PROML1, RP41, STGD4

Technical specifications

Antigen: CD133/1 (W6B3C1)
Clone: W6B3C1
Isotype: mouse IgG1κ
Isotype control: Mouse IgG1 – isotype control antibodies
Alternative names of antigen: PROM1, AC133, CORD12, MCDR2, MSTP061, PROML1, RP41, STGD4
Entrez Gene ID: 8842
Molecular mass of antigen [kDa]: 95
Distribution of antigen: brain, endothelial cells, epithelial cells, heart, hematopoietic stem cells, kidney, liver, lung, pancreas, placenta, ES and iPS cells, red blood cells
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: pure

Figure 1
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Figure 1

Protein extracts of human hippocampus (Lane 2), human fetal brain (Lane 3) and Weri-Rb-1 cells (Lane 5) were blotted onto a PVDF membrane and probed with CD133/1 (W6B3C1) pure antibody. Lane 4 is empty.

Goat anti-mouse IgG-HRP was applied at a dilution of 1:10,000 before bands were developed using ECL Plus™ chemiluminescent detection reagent. The molecular weight marker (Lane 1) indicates CD133 reactivity at approximately 120 kDa in Lanes 3 and 5.

Protein extracts of human hippocampus (Lane 2), human fetal brain (Lane 3) and Weri-Rb-1 cells (Lane 5) were blotted onto a PVDF membrane and probed with CD133/1 (W6B3C1) pure antibody. Lane 4 is empty.

Goat anti-mouse IgG-HRP was applied at a dilution of 1:10,000 before bands were developed using ECL Plus™ chemiluminescent detection reagent. The molecular weight marker (Lane 1) indicates CD133 reactivity at approximately 120 kDa in Lanes 3 and 5.
Figure 2
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Figure 2

Western blot analysis of 2×10
⁵
PBMCs (lane 1) spiked with 10% (lane 2), 5% (lane 3), 1% (lane 4), 0.5% (lane 5), and 0.2% (lane 6) WERI-Rb-1 cells, a CD133-expressing cell line. M: marker.

Western blot analysis of 2×10
⁵
PBMCs (lane 1) spiked with 10% (lane 2), 5% (lane 3), 1% (lane 4), 0.5% (lane 5), and 0.2% (lane 6) WERI-Rb-1 cells, a CD133-expressing cell line. M: marker.

Selected references
1. Yin, A. H. et al. (1997) AC133, a novel marker for human hematopoietic stem and progenitor cells. Blood 90: 5002-5012
2. Yu, S. et al. (2004)
  
  Isolation and characterization of the CD133
  ⁺
  precursors from the ventricular zone of human fetal brain by magnetic affinity cell sorting.
  
  Biotechnol. Lett. 26: 1131-1136
3. Belicchi, M. et al. (2004) Human skin-derived stem cells migrate throughout forebrain and differentiate into astrocytes after injection into adult mouse brain. J. Neurosci. Res. 77: 475-486
4. Richardson, G. et al. (2004) CD133, a novel marker for human prostatic epithelial stem cells. J. Cell. Sci. 117: 3539-3545
5. Alessandri, G. et al. (2004) Isolation and culture of human muscle-derived stem cells able to differentiate into myogenic and neurogenic cell lineages. Lancet 364: 1872-1883
6. Bussolati, B. et al. (2005) Isolation of renal progenitor cells from adult human kidney. Am. J. Pathol. 166: 545-555
7. Laurson, J. et al. (2005) Hepatocyte progenitors in man and in rodents--multiple pathways, multiple candidates. Int J Clin Exp Pathol 86: 1-18
8. Thill, M. et al. (2004)
  
  Identification of a population of CD133
  ⁺
  precursor cells in the stroma of human cornea.
  
  Invest. Ophthalmol. Vis. Sci. 45: 3519
9. Corbeil, D. et al. (2000) The human AC133 hematopoietic stem cell antigen is also expressed in epithelial cells and targeted to plasma membrane protrusions. J. Biol. Chem. 275: 5512-5520
10. Kaufman, D. et al. (2001) Hematopoietic colony-forming cells derived from human embryonic stem cells. Proc. Natl. Acad. Sci. U.S.A. 98: 10716-10721
11. Torrente et al. (2007)
  
  Autologous transplantation of muscle-derived CD133
  ⁺
  stem cells in Duchenne muscle patients.
  
  Cell Transplant 16: 563-577
12. Uchida, N. et al. (2000) Direct isolation of human central nervous system stem cells. Proc. Natl. Acad. Sci. U.S.A. 97: 14720-14725
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CD133/1 (W6B3C1) pure, human

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Technical specifications

Figure 1

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Figure 2

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Selected references

Brochures and posters

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