Alternative names

Technical specifications

• Antigen: CD54 (ICAM-1)
• Clone: REA266
• Isotype: recombinant human IgG1
• Isotype control: REA Control (S) antibodies
• Alternative names of antigen: ICAM-1, BB2, P3.58
• Entrez Gene ID: 3383
• Molecular mass of antigen [kDa]: 55
• Distribution of antigen: monocytes, macrophages, dendritic cells, endothelial cells, lymphocytes
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: PE, APC

• Figure 1

  Human peripheral blood mononuclear cells (PBMCs) were stained with CD54 (ICAM-1) antibodies as well as with CD19 antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

• Selected references

  1. Simmons, D. et al. (1988) ICAM, an adhesion ligand of LFA-1, is homologous to the neural cell adhesion molecule NCAM. Nature 331(6157): 624-627
  2. Greve, J. M. et al. (1989) The major human rhinovirus receptor is ICAM-1. Cell 56(5): 839-847
  3. Shimaoka, M. et al. (2003) Structures of the α L I domain and its complex with ICAM-1 reveal a shape-shifting pathway for integrin regulation. Cell 112(1): 99-111
  4. Vernot, J. P. et al. (2017)
     Phenotypic and functional alterations of hematopoietic stem and progenitor cells in an

     in vitro

     leukemia-induced microenvironment.
     Int J Mol Sci 18(2): E199