YILIMART - CD309 (VEGFR-2)-Biotin, human, 30 tests

The CD309 antibody recognizes the CD309 antigen, a transmembrane tyrosine kinase, and is also known as vascular endothelial growth factor receptor-2 (VEGFR-2) or kinase insert domain–containing receptor (KDR). CD309 is a high-affinity receptor for VEGF and plays a role in hematopoiesis. It is also involved in angiogenesis, both during embryogenesis and in the context of homeostatic or pathological events, e.g., vascularization of tumors. It has been identified on a subset of hematopoietic stem cells (HSCs), endothelial progenitor cells (EPCs), as well as on their common progenitor, the hemangioblast. CD309 expression is also found on mature endothelial cells (ECs).

Alternative names

KDR, Flk1, VEGFR, VEGFR2

Technical specifications

Antigen: CD309 (VEGFR-2)
Clone: ES8-20E6
Isotype: mouse IgG1κ
Isotype control: Mouse IgG1 – isotype control antibodies
Alternative names of antigen: KDR, Flk1, VEGFR, VEGFR2
Entrez Gene ID: 3791
Molecular mass of antigen [kDa]: 149
Distribution of antigen: endothelial cells, megakaryocytes, pancreas, platelets, ES and iPS cells, smooth muscle
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: VioBright FITC, PE, APC, Biotin

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were spiked with human umbilical vein endothelial cells (HUVECs), stained with CD309 (VEGFR-2) antibodies as well as with CD45 antibodies, and analyzed by flow cytometry using the MACSQuant

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Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

Selected references
1. Shalaby, F. et al. (1995) Failure of blood-island formation and vasculogenesis in Flk-1-deficient mice. Nature 376: 62-66
2. Eichmann, A. et al. (1997) Ligand-dependent development of the endothelial and hemopoietic lineages from embryonic Proc. Natl. Acad. Sci. U.S.A. 94: 5141-5146
3. Flamme, I. et al. (1995) Vascular endothelial growth factor (VEGF) and VEGF receptor 2 (flk-1) are expressed during vasculogenesis and vascular differentiation in the quail embryo. Dev. Biol. 169: 699-712
4. Auerbach, R. et al. (1996) Hematopoietic stem cells in the mouse embryonic yolk sac. Stem Cells 14: 269-280

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CD309 (VEGFR-2)-Biotin, human, 30 tests

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Alternative names

Technical specifications

Figure 1

Selected references

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