YILIMART - CD158a (KIR2DL1)-PE-Vio770, human, 30 t

Clone REA284 recognizes human CD158a (KIR2DL1), but not KIR2DL2, 2DL3, 2DL4, 2DL5, 2DS1, 2DS2, 2DS4, 3DL1, 3DL2, or 3DS1. CD158a is 58 kDa protein, also known as KIR2DL1. CD158a is a member of the family of killer cell immunoglobulin-like receptors (KIRs) comprising transmembrane glycoproteins expressed on NK cells and a subset of T cells. Many groups of KIRs exist including KIR3DL1-3, KIR3DS1, KIR2DL1-5, and KIR2DS1-5. The isoforms have either a short (S) or long (L) cytoplasmic domain which transduce either an activating or inhibitory signal, respectively. The ligands of CD158a (KIR2DL1) are HLA-C molecules with Asn77 and Lys80, but not Ser77 and Asn80.

Additional information: Clone REA284 displays negligible binding to Fc receptors.

Alternative names

KIR2DL1, KIR-K64, KIR221, NKAT, NKAT-1, p58.1

Technical specifications

Antigen: CD158a (KIR2DL1)
Clone: REA284
Isotype: recombinant human IgG1
Isotype control: REA Control (S) antibodies
Alternative names of antigen: KIR2DL1, KIR-K64, KIR221, NKAT, NKAT-1, p58.1
Entrez Gene ID: 3802
Molecular mass of antigen [kDa]: 36
Distribution of antigen: NK cells, T cells
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: FITC, APC-Vio770, PE, APC, PE-Vio770, Biotin

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were stained with CD158a (KIR2DL1) antibodies as well as with CD56 antibodies and analyzed by flow cytometry using the MACSQuant

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Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

Selected references
1. Valés-Gómez, M. et al. (1998) Kinetics of interaction of HLA-C ligands with natural killer cell inhibitory receptors. Immunity 9(3): 337-333
2. Wagtmann, N. et al. (1995) Molecular clones of the p58 NK cell receptor reveal immunoglobulin-related molecules with diversity in both the extra- and intracellular domains. Immunity 2(5): 439-449
3. Fan, Q. R. et al. (2001) Crystal structure of the human natural killer cell inhibitory receptor KIR2DL1-HLA-Cw4 complex. Nat. Immunol. 2(5): 452-460
4. Colonna, M. et al. (1995) Cloning of immunoglobulin-superfamily members associated with HLA-C and HLA-B recognition by human natural killer cells. Science 268(5209): 405-408
5. Jackson, E. et al. (2017) HIV exposed seronegative (HESN) compared to HIV infected individuals have higher frequencies of telomeric killer immunoglobulin-like receptor (KIR) B motifs; contribution of KIR B motif encoded genes to NK cell responsiveness. PLoS One 12(9): e0185160
6. Chapel, A. et al. (2017) Peptide-specific engagement of the activating NK cell receptor KIR2DS1. Sci. Rep. 7(1): 2414
7. Lu, W. et al. (2016)
  
  Suppression of HIV replication by CD8
  ⁺
  regulatory T-cells in elite controllers.
  
  Front Immunol 7: 134
Customer reports and application notes

Cross-reactivity of KIR antibodies

Flow cytometry analysis of whole-blood NK cells expressing single killer cell immunoglobulin–like receptors

CD158a (KIR2DL1)-PE-Vio770, human, 30 t

For research use only

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Alternative names

Technical specifications

Figure 1

Selected references

Customer reports and application notes

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