YILIMART - CD159c (NKG2C)-PE-Vio770, human, 100 t

Clone REA205 recognizes the CD159c antigen, a calcium-dependent (C-type) lectin which is also known as NKG2C type II integral membrane protein or KLRC2. CD159c heterodimerizes with CD94 to form a complex that recognizes HLA-E as ligand. Clone REA205 detects the human CD159c/CD94 heterodimer and does not cross-react with the human CD159a/CD94 heterodimer or with the human CD94 homodimer. CD159c is expressed primarily on natural killer (NK) cells and receptor ligation results in NK cell activation. The proportion of CD3

⁺

T cells expressing the CD159c/CD94 complex is elevated under pathologic conditions, such as human CMV infection, active celiac disease, paroxysmal nocturnal hemoglobinuria, Stevens-Johnson syndrome, and toxic epidermal necrolysis.

Additional information: Clone REA205 displays negligible binding to Fc receptors.

Alternative names

KLRC2, CD159c, NKG2-C, NKG2C

Technical specifications

Antigen: CD159c (NKG2C)
Clone: REA205
Isotype: recombinant human IgG1
Isotype control: REA Control (S) antibodies
Alternative names of antigen: KLRC2, CD159c, NKG2-C, NKG2C
Entrez Gene ID: 3822
Molecular mass of antigen [kDa]: 26
Distribution of antigen: NK cells
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: VioBright FITC, APC, PE, VioBright 515, PE-Vio770, Biotin, PE-Vio615

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were stained with CD159c (NKG2C) antibodies as well as with CD56 antibodies and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. The Tandem Signal Enhancer has been used to increase the binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

Selected references
1. Houchins, J. P. et al. (1997) Natural killer cell cytolytic activity is inhibited by NKG2-A and activated by NKG2-C. J. Immunol. 158(8): 3603-3609
2. Zaguia, F. et al. (2013)
  
  Cytotoxic NKG2C
  ⁺
  CD4 T cells target oligodendrocytes in multiple sclerosis.
  
  J. Immunol. 190(6): 2510-2518
3. Houchins, J. P. et al. (1991) DNA sequence analysis of NKG2, a family of related cDNA clones encoding type II integral membrane proteins on human natural killer cells. J. Exp. Med. 173(4): 1017-1020
4. Kruse, V. et al. (2015) Human induced pluripotent stem cells are targets for allogeneic and autologous natural killer (NK) cells and killing is partly mediated by the activating NK receptor DNAM-1. PLoS One 10(5): e0125544
5. López-Botet, M. et al. (1997) The CD94/NKG2C-type lectin receptor complex in recognition of HLA class I molecules. Res. Immunol. 148(3): 155-159
6. Hydes, T. et al. (2018) IL-12 and IL-15 induce the expression of CXCR6 and CD49a on peripheral natural killer cells. Immun. Inflamm. Dis. 6(1): 34-46
Customer reports and application notes

Comprehensive flow cytometry Analysis of isolated NK cells from whole blood, Temme, A. et al. (2019).

CD159c (NKG2C)-PE-Vio770, human, 100 t

For research use only

您浏览过的商品

Alternative names

Technical specifications

Figure 1

Selected references

Customer reports and application notes

相关产品