Alternative names

Technical specifications

• Antigen: TCR Vδ1
• Clone: REA173
• Isotype: recombinant human IgG1
• Isotype control: REA Control (S) antibodies
• Alternative names of antigen: TRDV1, hDV101S1
• Entrez Gene ID: 28518
• Distribution of antigen: T cells, epithelial cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: FITC, APC, PE-Vio770, PE, VioBlue, VioGreen, APC-Vio770, PerCP-Vio700, Biotin

• Figure 1

  Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-TCR Vδ1 antibodies as well as with CD3 antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. The specificity of the conjugated antibodies was confirmed by blocking the binding to the ligand, using pure unconjugated antibodies (left images). The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

• Selected references

  1. Di Marco Barros, R. et al. (2016) Epithelia use butyrophilin-like molecules to shape organ-specific γδ T cell compartments. Cell 167(1): 203-218
  2. Libero, G. D. et al. (1997) Sentinel function of broadly reactive human gamma delta T cells. Immunol. Today 18(1): 22-26
  3. Pepin, L. F. et al. (1993) Preferential V delta 1 expression among TcR gamma/delta-bearing T cells in human oral epithelium. Scand. J. Immunol. 37(3): 289-294
  4. Siegers, G. et al. (2018) Functional plasticity of gamma delta T cells and breast tumor targets in hypoxia. Front Immunol 9
  5. Davey, M. S. et al. (2017) Clonal selection in the human Vδ1 T cell repertoire indicates γδ TCR-dependent adaptive immune surveillance. Nat Commun 8: 14760
  6. Davey, M. et al. (2018) The human Vδ2+ T-cell compartment comprises distinct innate-like Vγ9+ and adaptive Vγ9- subsets. Nat Commun 9(1)
  7. Hunter, S. et al. (2018) Human liver infiltrating γδ T cells are composed of clonally expanded circulating and tissue-resident populations. J. Hepatol. 69(3)
  8. Zumwalde, N. A. et al. (2016)
     Analysis of immune cells from human mammary ductal epithelial organoids reveals Vδ2

     ⁺

     T cells that efficiently target breast carcinoma cells in the presence of bisphosphonate.
     Cancer Prev Res (Phila) 9: 305-316