Alternative names

Technical specifications

• Antigen: CD279 (PD1)
• Clone: REA1165
• Isotype: recombinant human IgG1
• Isotype control: REA Control (S) antibodies
• Alternative names of antigen: PDCD1, PD1, SLEB2, hPD-1, hPD-l, hSLE1
• Entrez Gene ID: 5133
• Molecular mass of antigen [kDa]: 29
• Cross-reactivity:
  rhesus monkey (

  Macaca mulatta

  )
• Distribution of antigen: B cells, monocytes, spleen, T cells, thymocytes
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: PE, PE-Vio770, VioBright 515, Biotin

• Figure 1

  Human peripheral blood mononuclear cells (PBMCs) were stained with CD279 (PD1) antibodies or with the corresponding REA Control (S) antibodies (left images) as well as with CD4 antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

• Selected references

  1. Okazaki, T. and Honjo, T. (2007) PD-1 and PD-1 ligands: from discovery to clinical application. Int. Immunol. 19: 813-824
  2. Keir, M. E. et al. (2008) PD-1 and its ligands in tolerance and immunity. Annu. Rev. Immunol. 26: 677-704
  3. Serriari, N.-E. et al. (2010) B and T lymphocyte attenuator is highly expressed on CMV-specific T cells during infection and regulates their function. J. Immunol. 185: 3140-3148
  4. Fife, B. T. et al. (2011) The role of the PD-1 pathway in autoimmunity and peripheral tolerance. Ann. N. Y. Acad. Sci. 1217: 45-59

• Scientific posters

  Flow cytometric assays with automated data acquisition and analysis for CAR T cell manufacturing and patient immunomonitoring. Mues et al. CIMT (2019).