YILIMART - CD45R (B220)-PE, mouse, RA3-6B2, 100 t

Clone RA3-6B2 recognizes the mouse CD45R (B220) antigen which is expressed on B lymphocytes throughout their development from early pro–B stages onwards and is down-regulated upon terminal differentiation to plasma cells. Apart from B cells, CD45R is expressed on a small subset of dendritic cells (plasmacytoid dendritic cells). The CD45R monoclonal antibody clone RA3-6B2 specifically recognizes the exon A–restricted isoform of mouse CD45. CD45R is absent in thymus but reported to be present on apoptotic thymocytes.

Alternative names

Ptprc, B220, CD45, loc, L-CA, Ly-5, Lyt-4, T200

Technical specifications

Antigen: CD45R (B220)
Clone: RA3-6B2
Isotype: rat IgG2aκ
Isotype control: Rat IgG2a – isotype control antibodies
Alternative names of antigen: Ptprc, B220, CD45, loc, L-CA, Ly-5, Lyt-4, T200
Entrez Gene ID: 19264
Molecular mass of antigen [kDa]: 142
Distribution of antigen: B cells, NK cells, T cells
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: FITC, VioBlue, VioGreen, PerCP, PE, APC, PE-Vio615, Biotin

Figure 1

Mouse splenocytes were stained with CD45R (B220) antibodies and analyzed by flow cytometry using the MACSQuant

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Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

CD45R (B220)-PE, mouse, RA3-6B2, 100 t

For research use only

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Alternative names

Technical specifications

Figure 1

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