Alternative names

Technical specifications

• Antigen: CD159a (NKG2A)
• Clone: REA110
• Isotype: recombinant human IgG1
• Isotype control: REA Control (S) antibodies
• Alternative names of antigen: KLRC1, CD159a, NKG2, NKG2A
• Entrez Gene ID: 3821
• Molecular mass of antigen [kDa]: 26
• Cross-reactivity:
  chimpanzee (

  Pan troglodytes

  )
  ,
  olive baboon (

  Papio anubis

  )
  ,
  rhesus monkey (

  Macaca mulatta

  )
  ,
  cynomolgus monkey (

  Macaca fascicularis

  )
• Distribution of antigen: NK cells, T cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: FITC, VioBright FITC, PE, APC, PE-Vio770, Biotin, PE-Vio615

• Figure 1

  Human peripheral blood mononuclear cells (PBMCs) were stained with CD159a (NKG2A) antibodies as well as with CD56 antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

• Selected references

  1. Rueda, C. et al. (2016)
     Lipopolysaccharide-induced chorioamnionitis promotes IL-1-dependent inflammatory FOXP3

     ⁺

     CD4

     ⁺

     T cells in the fetal rhesus macaque.
     J. Immunol. 196(9): 3706-3715
  2. Boyington, J. C. et al. (1999) Structure of CD94 reveals a novel C-type lectin fold: implications for the NK cell-associated CD94/NKG2 receptors. Immunity 10(1): 75-82
  3. Sullivan, L. C. et al. (2007) The heterodimeric assembly of the CD94-NKG2 receptor family and implications for human leukocyte antigen-E recognition. Immunity 27(6): 900-911
  4. Reeves R. K. et al. (2015) Antigen-specific NK cell memory in rhesus macaques. Nat. Immunol. 16: 927-932
  5. Veluchamy, J. P. et al. (2017) High-efficiency lysis of cervical cancer by allogeneic NK cells derived from umbilical cord progenitors is independent of HLA status. Cancer Immunol. Immunother. 66: 51-61
  6. Wolpert, F. et al. (2015) Interferon-β modulates the innate immune response against glioblastoma initiating cells. PLoS One 10(10): e0139603
  7. Borrego, F. et al. (2006) The CD94/NKG2 family of receptors: from molecules and cells to clinical relevance. Immunol. Res. 35(3): 263-278
  8. Croci, S. et al. (2018) Higher frequencies of lymphocytes expressing the natural killer group 2D receptor in patients with Behçet disease. Front Immunol 9: 2157
  9. Jacomet, F. et al. (2017)
     The hypothesis of the human iNKT/innate CD8

     ⁺

     T-cell axis applied to cancer: evidence for a deficiency in chronic myeloid leukemia.
     Front Immunol 7: 688
  10. Mahaweni, N. et al. (2018)
      NKG2A expression is not

      per se

      detrimental for the anti-multiple myeloma activity of activated natural killer cells in an

      in vitro

      system mimicking the tumor microenvironment.
      Front Immunol 9: 1415
  11. Rechtien, A. et al. (2017) Systems vaccinology identifies an early innate immune signature as a correlate of antibody responses to the Ebola vaccine rVSV-ZEBOV. Cell Rep 20(9): 2251-2261

• Customer reports and application notes

  Comprehensive flow cytometry Analysis of isolated NK cells from whole blood, Temme, A. et al. (2019).
  Flow cytometry analysis of whole-blood NK cells expressing single killer cell immunoglobulin–like receptors