YILIMART - CD19-VioBlue, human, REA675, 100 tests

REA675 recognizes the human CD19 antigen, a type I transmembrane glycoprotein of 95 kDa that belongs to the immunglobulin superfamily. CD19 is expressed on B cells throughout most stages of B cell differentiation, though its expression is down-regulated during their terminal differentiation to plasma cells. Expression of CD19 is also found in the majority of B cell–derived malignancies. CD19 is further present on follicular dendritic cells. On B cells, CD19 associates with CD21, CD81, and CD225 (Leu-13) forming a signal transduction complex.

Additional information: Clone REA675 displays negligible binding to Fc receptors.

Alternative names

B4, Leu-12

Technical specifications

Antigen: CD19
Clone: REA675
Isotype: recombinant human IgG1
Isotype control: REA Control (S) antibodies
Alternative names of antigen: B4, Leu-12
Distribution of antigen: B cells, dendritic cells
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: PE-Vio770, APC-Vio770, PerCP-Vio700, VioBright 515, Biotin, FITC, PE, APC, VioBlue, VioGreen, PE-Vio615

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were stained with CD19 antibodies or with the corresponding REA Control (S) antibodies (left images) as well as with CD20 antibodies. Flow cytometry was performed using the MACSQuant

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Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

Selected references
1. Nadler, L. M. et al. (1983) B4, a human B lymphocyte-associated antigen expressed on normal, mitogen-activated, and malignant B lymphocytes. J. Immunol. 131(1): 244-250
2. Fujimoto, M. et al. (2000) CD19 regulates intrinsic B lymphocyte signal transduction and activation through a novel mechanism of processive amplification. Immunol. Res. 22(2-3): 281-298
3. Poe, J. C. et al. (2001) CD19, CD21, and CD22: multifaceted response regulators of B lymphocyte signal transduction. Int. Rev. Immunol. 20(6): 739-762
4. Tedder, T. F. et al. (2002) CD19-CD21 complex regulates an intrinsic Src family kinase amplification loop that links innate immunity with B-lymphocyte intracellular calcium responses. Biochem. Soc. Trans. 30(4): 807-811
Scientific posters

Increased sensitivity of flow cytometry for detection of minimal residual disease and circulating tumor cells in B Non-Hodgkin Lymphoma

CD19-VioBlue, human, REA675, 100 tests

For research use only

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Alternative names

Technical specifications

Figure 1

Selected references

Scientific posters

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