Sample Preparation
Cell Separation
Flow Cytometry
Cell Culture&Stimulation
Cell manufacturing
Imaging and Microscopy
MACSmolecular
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Human peripheral blood mononuclear cells (PBMCs), either unstimulated (left images) or stimulated overnight with PepTivator ® EBV Consensus, were stained with CD137 antibodies as well as with CD8 antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates. |