YILIMART - CD366 (TIM-3)-APC, human, REA635, 30 t

Clone REA635 recognizes the human CD366 antigen, a single-pass type I membrane protein, which is also known as T cell immunoglobulin and mucin domain containing protein-3 (TIM-3) or Hepatitis A virus cellular receptor 2 (HAVcr-2). CD366 is expressed on activated T cells, especially Tʜ1 cells, monocytes, macrophages, and dendritic cells. It is an activation-induced inhibitory molecule involved in tolerance and shown to induce T cell exhaustion in chronic viral infection and cancers. CD366 has been proposed to inhibit Tʜ1-mediated immune responses and regulates Tʜ1 and Tʜ17 cytokine secretion. The interaction of CD366 and its ligand galectin-9 induces apoptosis of Tʜ1 cells.

Additional information: Clone REA635 displays negligible binding to Fc receptors.

Alternative names

HAVcr-2, TIMD-3

Technical specifications

Antigen: CD366 (TIM-3)
Clone: REA635
Isotype: recombinant human IgG1
Isotype control: REA Control (S) antibodies
Alternative names of antigen: HAVcr-2, TIMD-3
Entrez Gene ID: 84868
Molecular mass of antigen [kDa]: 31
Cross-reactivity:

rhesus monkey (
Macaca mulatta
)
Distribution of antigen: dendritic cells, macrophages, monocytes, T cells
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: PE, APC, PE-Vio770

Figure 1

Human peripheral blood mononuclear cells (PBMCs), either left unstimulated (left images) or stimulated with CD3/CD28 antibodies for 3 days, were stained with CD366 (TIM-3) antibodies as well as with CD4 antibodies. Cells were analyzed by flow cytometry using the MACSQuant

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Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.