YILIMART - CD312 (EMR2)-PE, human, REA302, 30 tests

Clone REA302 recognizes the human CD312 antigen, a multi-pass membrane protein also known as EGF-like module-containing mucin-like hormone receptor-like 2 (EMR2) which shares strikingly similar molecular characteristics with CD97. CD312 is a member of the epidermal growth factor-seven transmembrane (EGF-TM7) family of proteins expressed predominantly by cells of the immune system. CD312 fails to interact with CD55, the cellular ligand for CD97, suggesting the possibility of different cellular ligands. CD312 interacts with chondroitin sulphate glycosaminoglycans in an isoform-specific manner. Expression of CD312 is up-regulated during differentiation and maturation of macrophages, and is conversely down-regulated during dendritic cell maturation. In monocytes and macrophages, CD312 can be specifically up-regulated by LPS and interleukin 10 (IL-10) via an IL-10–mediated pathway. In inflamed tissues, CD312 is detected in subpopulations of myeloid cells including macrophages and neutrophils. CD312 plays a role in the migration and adhesion of myeloid cells during cell differentiation, maturation, and activation.

Additional information: Clone REA302 displays negligible binding to Fc receptors.

Alternative names

ADGRE2, EMR2

Technical specifications

Antigen: CD312 (EMR2)
Clone: REA302
Isotype: recombinant human IgG1
Isotype control: REA Control (S) antibodies
Alternative names of antigen: ADGRE2, EMR2
Entrez Gene ID: 30817
Molecular mass of antigen [kDa]: 88
Distribution of antigen: myeloid cells, neutrophils
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: PE, APC

Figure 1

Human peripheral blood cells after erythrocyte lysis were stained with CD312 (EMR2) antibodies or with the corresponding REA Control (S) antibodies (left images) as well as with CD14 antibodies and analyzed by flow cytometry using the MACSQuant

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Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

Selected references
1. Kwakkenbos, M. J. et al. (2002) The human EGF-TM7 family member EMR2 is a heterodimeric receptor expressed on myeloid cells. J. Leukoc. Biol. 71(5): 854-862
2. Chang, G. W. et al. (2007) CD312, the human adhesion-GPCR EMR2, is differentially expressed during differentiation, maturation, and activation of myeloid cells. Biochem. Biophys. Res. Commun. 353(1): 133-138
3. Yona, S. et al. (2008) Ligation of the adhesion-GPCR EMR2 regulates human neutrophil function. FASEB J. 22(3): 741-751

CD312 (EMR2)-PE, human, REA302, 30 tests

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Alternative names

Technical specifications

Figure 1

Selected references

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