Alternative names

Technical specifications

• Antigen: CD166
• Clone: REA442
• Isotype: recombinant human IgG1
• Isotype control: REA Control (S) antibodies
• Alternative names of antigen: ALCAM, MEMD
• Entrez Gene ID: 214
• Molecular mass of antigen [kDa]: 62
• Distribution of antigen: hematopoietic stem cells, T cells, B cells, monocytes, melanoma, endothelial cells, mesenchymal stem cells, osteoblasts
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: PE, APC, VioBright FITC

• Figure 1

  Human peripheral blood mononuclear cells (PBMCs) were stained with CD166 antibodies or with the corresponding REA Control (S) antibodies (left images) as well as with CD14 antibodies. Flow cytometry was performed using the MACSQuant ® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

• Selected references

  1. Chitteti, B. R. et al. (2014) CD166 regulates human and murine hematopoietic stem cells and the hematopoietic niche. Blood 124(4): 519-529
  2. Degen, W. G. et al. (1998) MEMD, a new cell adhesion molecule in metastasizing human melanoma cell lines, is identical to ALCAM (activated leukocyte cell adhesion molecule). Am. J. Pathol. 152(3): 805-813
  3. Ni, C. et al. (2013) Prognostic value of CD166 expression in cancers of the digestive system: a systematic review and meta-analysis. PLoS One 8(8): e70958