YILIMART - CD3-APC-Vio770, mouse, REA641, 100 tests

Clone REA641 recognizes the mouse CD3 complex, which is expressed on all mature T lymphocytes, NKT cells, and during the development of thymocytes. CD3, also known as T3, belongs to the Ig superfamily. It consists of CD3ε, δ, γ, and ζ chains and forms a TCR complex by associating with TCR α/β or γ/δ chains. The TCR does not possess intracellular signaling domains, uncoupling Ag recognition from T cell signaling. The TCR is instead non-covalently associated with a multisubunit signaling apparatus, consisting of the CD3εγ and CD3εδ heterodimers and the CD3ζζ homodimer, which collectively form the TCR-CD3 complex. CD3 is involved in T cell activation, signal transduction, and antigen recognition by binding the peptide/MHC antigen complex.

Additional information: Clone REA641 displays negligible binding to Fc receptors.

Alternative names

T cell antigen receptor complex, T3, AI504783, CD3epsilon, T3E, Ctg-3, Ctg3, T3g

Technical specifications

Antigen: CD3
Clone: REA641
Isotype: recombinant human IgG1
Isotype control: REA Control antibodies
Alternative names of antigen: T cell antigen receptor complex, T3, AI504783, CD3epsilon, T3E, Ctg-3, Ctg3, T3g
Entrez Gene ID: 12501, 12502
Molecular mass of antigen [kDa]: 37
Distribution of antigen: NKT cells, T cells, thymocytes
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: PE-Vio770, FITC, APC, APC-Vio770, PerCP-Vio700, PE, Biotin

Figure 1

Splenocytes from C57BL/6 mice were stained with CD3 antibodies or with the corresponding REA Control antibodies (left images) as well as with CD8a antibodies. Flow cytometry was performed using the MACSQuant

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Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

Selected references
1. Birnbaum, M. E. et al. (2014) Molecular architecture of the αβ T cell receptor-CD3 complex. Proc. Natl. Acad. Sci. U.S.A. 111(49): 17576-17581
2. Gold, D. P. et al. (1987) Evolutionary relationship between the T3 chains of the T-cell receptor complex and the immunoglobulin supergene family. Proc. Natl. Acad. Sci. U.S.A. 84(21): 7649-7653
3. Miescher, G. C. et al. (1989) Production and characterization of a rat monoclonal antibody against the murine CD3 molecular complex. Immunol. Lett. 23(2): 113-118
4. Exley, M. et al. (1991) Structure, assembly and intracellular transport of the T cell receptor for antigen. Semin. Immunol. 3(5): 283-297
Scientific posters

Analysis of T cells made easy – Complete workflows improve the isolation and analysis of T cells. Evaristo et al. AAI (2019).

CD3-APC-Vio770, mouse, REA641, 100 tests

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Alternative names

Technical specifications

Figure 1

Selected references

Scientific posters

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