YILIMART - CD81-APC, human, REA513, 100 tests

Clone REA513 recognizes the human CD81 antigen, a multi-pass membrane protein also known as TAPA-1. It is a member of the tetraspanin family including, for example, CD9, CD37, CD53, CD63, CD82, and CD151. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth, and motility. The 26 kDa cell surface protein is involved in adhesion, activation, proliferation, and differentiation of B cells, T cells, and other cells. On B cells CD81 is part of a complex with CD21, CD19, and Leu13. Similarly on T cells CD81 associates with CD4 and CD8 and provides a costimulatory signal with CD3. CD81 is expressed by epithelial and endothelial cells, T and B cells, as well as natural killer (NK) cells.

Additional information: Clone REA513 displays negligible binding to Fc receptors.

Alternative names

CVID6, S5.7, TAPA1, TSPAN28

Technical specifications

Antigen: CD81
Clone: REA513
Isotype: recombinant human IgG1
Isotype control: REA Control (S) antibodies
Alternative names of antigen: CVID6, S5.7, TAPA1, TSPAN28
Entrez Gene ID: 975
Molecular mass of antigen [kDa]: 26
Cross-reactivity:

chimpanzee (
Pan troglodytes
)
Distribution of antigen: epithelial cells, endothelial cells, T cells, B cells, NK cells
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: PE, APC, Biotin

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were stained with CD81 antibodies or with the corresponding REA Control (S) antibodies (left images). Flow cytometry was performed using the MACSQuant

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Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

Selected references
1. Levy, S. (1998) CD81 (TAPA-1): a molecule involved in signal transduction and cell adhesion in the immune system. Annu. Rev. Immunol. 16: 89-109
2. Wright, M. D. et al. (1994) The ins and outs of the transmembrane 4 superfamily. Immunol. Today 15(12): 588-594
3. Levy, S. (1991) Structure and membrane topology of TAPA-1. J. Biol. Chem. 266(22): 14597-14602
4. Oren, R. (1990) TAPA-1, the target of an antiproliferative antibody, defines a new family of transmembrane proteins. Mol. Cell. Biol. 10(8): 4007-4015

CD81-APC, human, REA513, 100 tests

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Alternative names

Technical specifications

Figure 1

Selected references

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