Alternative names

Technical specifications

• Antigen: CD96 (TACTILE)
• Clone: REA195
• Isotype: recombinant human IgG1
• Isotype control: REA Control (S) antibodies
• Alternative names of antigen: TACTILE
• Distribution of antigen: T cells, NK cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: PE-Vio770, APC

• Figure 1

  Human peripheral blood mononuclear cells (PBMCs) were stained with CD96 (TACTILE) antibodies as well as with CD19 antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. The specificity of the conjugated antibodies was confirmed by blocking the binding to the ligand, using pure unconjugated antibodies (left images). The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

• Selected references

  1. Fuchs, A. et al. (2004) Cutting Edge: CD96 (Tactile) promotes NK cell-target cell adhesion by interacting with the poliovirus receptor (CD155). J. Immunol. 172: 3994-3998
  2. Meyer, D. et al. (2009) CD96 interaction with CD155 via its first Ig-like domain is modulated by alternative splicing or mutations in distal Ig-like domains. J. Biol. Chem. 284: 2235-2244