YILIMART - CD94-PerCP-Vio700, human, REA113, 100 t

The clone REA113 recognizes CD94, an approximately 45 kDa, single-pass type II transmembrane protein, which belongs to the C-type lectin superfamily. CD94 associates covalently with NKG2 family members and forms disulfide bonded heterodimers. CD94-NKG2 receptors bind HLA-E, the non-classical MHC class Ib molecule. Depending on the associated NKG2 member, CD94-NKG2 heterodimer transmits either activating or inhibitory signal. For, e.g, via immunoreceptor tyrosine based-inhibitory motifs (ITIMs) containing NKG2A, CD94-NKG2A heterodimers inhibit NK cell cytotoxicity. Besides NK cells, expression of CD94-NKG2 heterodimers is also shown on NK-T cells and a small percentage of CD8 cells in mice, where the crosslinking of CD94-NKG2 was shown to reduce the level of spontaneous apoptosis.

Additional information: Clone REA113 displays negligible binding to Fc receptors.

Alternative names

KLRD1, Kp43

Technical specifications

Antigen: CD94
Clone: REA113
Isotype: recombinant human IgG1
Isotype control: REA Control (S) antibodies
Alternative names of antigen: KLRD1, Kp43
Entrez Gene ID: 3824
Molecular mass of antigen [kDa]: 21
Distribution of antigen: NK cells
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: PerCP-Vio700, APC-Vio770

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were stained with CD94 antibodies as well as with CD56 antibodies and analyzed by flow cytometry using the MACSQuant

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Analyzer. Lymphocytes were gated for the analysis. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

Selected references
1. Gunturi, A. et al. (2003) Preferential survival of CD8 T and NK cells expressing high levels of CD94. J. Immunol. 170: 1737-1745
2. Braud, V. M. et al. (1998) HLA-E binds to natural killer cell receptors CD94/NKG2A, B and C. Nature 391(6669): 795-799
3. Brooks, A. G. et al. (1997) NKG2A complexed with CD94 defines a novel inhibitory natural killer cell receptor. J. Exp. Med. 185: 795-800
4. Jeffery, H. et al. Human intrahepatic ILC2 are IL-13positive amphiregulinpositive and their frequency correlates with model of end stage liver disease score. PLoS One 12(12): e0188649
5. Wolpert, F. et al. (2015) Interferon-β modulates the innate immune response against glioblastoma initiating cells. PLoS One 10(10): e0139603
6. Canté-Barrett, K. et al. (2017)
  
  Loss of CD44
  ^dim
  expression from early progenitor cells marks T-cell lineage commitment in the human thymus.
  
  Front Immunol 8: 32

CD94-PerCP-Vio700, human, REA113, 100 t

For research use only

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Alternative names

Technical specifications

Figure 1

Selected references

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