YILIMART - CD20-PE-Vio615, human, LT20, 30 tests

The antibody recognizes the CD20 antigen, a non-glycosylated transmembrane protein of 33–37 kDa that is expressed on B lineage cells from the pre–B cell stage to the B cell lymphoblast stage. The antigen is further expressed on most malignant B cells. CD20 is not found on early B cell progenitors or plasma cells. Oligomers of CD20 form a Ca

²⁺

channel and might function in the regulation of local responses during B cell activation.

Alternative names

MS4A1, B1, Bp35, CVID5, LEU-16, MS4A2, S7, Ly-44

Technical specifications

Antigen: CD20
Clone: LT20
Isotype: mouse IgG1κ
Isotype control: Mouse IgG1 – isotype control antibodies
Alternative names of antigen: MS4A1, B1, Bp35, CVID5, LEU-16, MS4A2, S7, Ly-44
Entrez Gene ID: 931
Molecular mass of antigen [kDa]: 33
Cross-reactivity:

rhesus monkey (
Macaca mulatta
)

,

cynomolgus monkey (
Macaca fascicularis
)
Distribution of antigen: B cells, cancer stem cells, dendritic cells, lymphocytes
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: FITC, PE, APC, VioBlue, VioGreen, PerCP, PE-Vio770, APC-Vio770, PerCP-Vio700, Biotin, PE-Vio615

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were stained with CD20 antibodies and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

Figure 2
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Figure 2

Rhesus monkey PBMCs were stained with CD20-FITC and analyzed by flow cytometry.

Rhesus monkey PBMCs were stained with CD20-FITC and analyzed by flow cytometry.
Figure 3
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Figure 3

Cynomolgus monkey PBMCs were stained with CD20-FITC and analyzed by flow cytometry.

Cynomolgus monkey PBMCs were stained with CD20-FITC and analyzed by flow cytometry.

Selected references
1. Lamprecht, B. et al. (2008) Aberrant expression of the Tʜ2 cytokine IL-21 in Hodgkin lymphoma cells regulates STAT3 signaling and attracts Treg cells via regulation of MIP-3alpha. Blood 112(8): 3339-3347
2. Maxwell, S. A. et al. (2009) 14-3-3zeta J. Biol. Chem. 284(33): 22379-22389
3. Countouriotis, T. B. et al. (2002) Cell surface antigen and molecular targeting in the treatment of hematologic malignancies. Stem Cells 20(3): 215-229
4. Cragg, M. S. et al. (2002)
  
  Opposing properties of CD20 mAb; in: Mason, D.
  et al.
  (eds.): Leucocyte typing VII,
  
  Oxford, Oxford University Press
5. Polyak, M. J. and Deans, J. P. (2002)
  
  CD20 Workshop Panel report: in Mason, D.
  et al.
  (eds.): Leucocyte typing VII,
  
  Oxford, Oxford University Press

CD20-PE-Vio615, human, LT20, 30 tests

For research use only

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Selected references

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