Alternative names

Technical specifications

• Antigen: TCRα/β
• Clone: REA652
• Isotype: recombinant human IgG1
• Isotype control: REA Control (S) antibodies
• Alternative names of antigen: TCR a/b, IMD7, TCRA, TRAC, TRA, TRB
• Entrez Gene ID: 6955, 6957
• Distribution of antigen: T cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: FITC, PE, APC, APC-Vio770, PerCP-Vio700, Biotin, VioBlue, VioGreen, PE-Vio770

• Figure 1

  Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-TCRα/β antibodies or with the corresponding REA Control (S) antibodies (left peak). Flow cytometry was performed using the MACSQuant ® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

• Selected references

  1. Brenner, M. B. et al. (1986) Identification of a putative second T-cell receptor. Nature 322(6075): 145-149
  2. Call, M. E. et al. (2002) The organizing principle in the formation of the T cell receptor-CD3 complex. Cell 111(7): 967-979
  3. Oettgen, H. C. et al. (1984) Characterization of the two heavy chains of the T3 complex on the surface of human T lymphocytes. J. Biol. Chem. 259(19): 12039-12048