Alternative names

Technical specifications

• Antigen: CD51/CD61
• Clone: REA1099
• Isotype: recombinant human IgG1
• Isotype control: REA Control (S) antibodies
• Alternative names of antigen: ITGAV, MSK8, VNRA, VTNR, BDPLT16, BDPLT2, CD61, GP3A, GPIIIa, ITGB3
• Entrez Gene ID: 3685, 3690
• Molecular mass of antigen [kDa]: 197
• Cross-reactivity: rabbit, chicken
• Distribution of antigen: endothelial cells, melanoma, osteoclasts, platelets
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: PE, APC, PE-Vio770, VioBright 515

• Figure 1

  SK-MEL-28 cells were stained with CD51/CD61 antibodies or with the corresponding REA Control (S) antibodies (left peak) and analyzed by flow cytometry using the MACSQuant ® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

• Selected references

  1. Athanasou, N. A. et al. (1990) New sites of cellular vitronectin receptor immunoreactivity detected with osteoclast-reacting monoclonal antibodies 13C2 and 23C6. Bone Miner. 8(1): 7-22
  2. Chuntharapai, A. et al. (1993) Blocking monoclonal antibodies to alpha V beta 3 integrin: a unique epitope of alpha V beta 3 integrin is present on human osteoclasts. Exp. Cell Res. 205(2): 345-352