Alternative names

Technical specifications

• Antigen: MICA/MICB
• Clone: REA1076
• Isotype: recombinant human IgG1
• Isotype control: REA Control (S) antibodies
• Alternative names of antigen: MICB, PERB11.2, MICA, PERB11.1
• Entrez Gene ID: 100507436, 4277
• Molecular mass of antigen [kDa]: 41
• Distribution of antigen: endothelial cells, epithelial cells, fibroblasts, NK cells, T cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: PE, APC, PE-Vio615, PE-Vio770, PerCP-Vio700, VioBright 515, Biotin

• Figure 1

  Hela cells were stained with Anti-MICA/MICB antibodies or with the corresponding REA Control (S) antibodies (left peak) and analyzed by flow cytometry using the MACSQuant ® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

• Selected references

  1. Houchins, J. P. et al. (1991) DNA sequence analysis of NKG2, a family of related cDNA clones encoding type II integral membrane proteins on human natural killer cells. J. Exp. Med. 173(4): 1017-1020
  2. Bauer, S. et al. (1999) Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA. Science 285: 727-729
  3. Li, P. et al. (1999) Crystal structure of the MHC class I homolog MIC-A, a γδ T cell ligand. Immunity 10: 577-584