YILIMART - CD19 CAR Detection Reagent (Biotin), human

The CD19 CAR Detection Reagent (Biotin) has been developed for the detection of transduced T cells that are engineered to express CD19-specific chimeric antigen receptors (CAR) on the cell surface, which recognize human CD19 antigen.

The CD19 CAR Detection Reagent (Biotin) is an antigen based detection reagent conjugated to biotin. It contains a recombinantly expressed fusion protein consisting of the human CD19 extracellular domains and a specifically mutated human IgG1 Fc region.

The engineered CD19 CAR T cells can be detected via the recognition of the CD19 protein, and identified by flow cytometry via anti-biotin fluorochromes. The mutated human IgG1 Fc region of the CD19 CAR Detection Reagent abolishes their binding to Fcγ receptors. This allows for background-free analysis and eliminates the need for additional blocking steps, such as using a FcR blocking reagent.

Technical specifications

Antigen: CD19 CAR
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: Biotin

Figure 1

CD19 CAR

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T cells, generated using the CliniMACS Prodigy

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TCT process within 12 days, were stained with CD19 CAR Detection Reagent (Biotin), Anti-Biotin-PE, CD3-FITC, and 7-AAD Staining Solution and analyzed by flow cytometry using the MACSQuant

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Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and 7-AAD fluorescence. Shown are viable CD3

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T cells.

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CD19 CAR Detection Reagent (Biotin), human

For research use only

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Technical specifications

Figure 1

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