Alternative names

Technical specifications

• Antigen: TCRγ/δ
• Clone: REA591
• Isotype: recombinant human IgG1
• Isotype control: REA Control (S) antibodies
• Alternative names of antigen: TCRgd
• Entrez Gene ID: 6965
• Distribution of antigen: T cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: FITC, PE, APC, PE-Vio770, APC-Vio770, PerCP-Vio700, Biotin

• Figure 1

  Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-TCRγ/δ antibodies or with the corresponding REA Control (S) antibodies (left images) as well as with CD3 antibodies. Flow cytometry was performed using the MACSQuant ® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

• Selected references

  1. Airoldi, I. et al. (2015)
     γδ T-cell reconstitution after HLA-haploidentical hematopoietic transplantation depleted of TCR-αβ

     ⁺

     /CD19

     ⁺

     lymphocytes.
     Blood 125(15): 2349-2358
  2. Bonneville, M. et al. (2010) Gammadelta T cell effector functions: a blend of innate programming and acquired plasticity. Nat. Rev. Immunol. 10(7): 467-478
  3. Wiest, D. L. (2016) Development of γδ T cells, the special-force soldiers of the immune system. Methods Mol. Biol. 1323: 23-32