YILIMART - Anti-TCRg/d-Biotin, human, 11F2, 30 t

The T cell receptor is a heterodimeric glycoprotein associated with the CD3 antigen. The TCR consists of an α and a β chain (TCRα/β) or a γ and a δ chain (TCRγ/δ). Clone 11F2 reacts with a framework epitope of the γ/δ T cell–receptor. The γ and δ TCR chains are composed of constant and variable regions, each encoded by distinct gene segments. The γ chain forms either disulfide-linked or non-disulfide-linked heterodimers with the δ-subunit.The γ/δ Tcell–receptor is present on a subset of T lymphocytes in peripheral blood, intestinal epithelium, lymph node, thymus, and spleen. TCR γ/δ is involved in the recognition of certain bacterial, self-CD1 molecule, and tumor antigens bound to MHC class I. γ/δ T cells are mainly CD4 negative and CD8 negative. T cells expressing the γ/δ TCR have been shown to play a role in oral tolerance, innate immune response for some tumor cells, and autoimmune disease. Antigen presentation by γ/δ T cells has been reported.

Alternative names

TRD, TCRD, TCRDV1, TRD@, TCRG, TRG@, TRG

Technical specifications

Antigen: TCRγ/δ
Clone: 11F2
Isotype: mouse IgG1κ
Isotype control: Mouse IgG1 – isotype control antibodies
Alternative names of antigen: TRD, TCRD, TCRDV1, TRD@, TCRG, TRG@, TRG
Entrez Gene ID: 6965
Distribution of antigen: T cells
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: FITC, PE, APC, VioBlue, PE-Vio770, APC-Vio770, PerCP-Vio700, Biotin

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-TCRγ/δ antibodies as well as with CD3 antibodies and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

Selected references
1. Spencer, C. T. et al. (2008) Only a subset of phosphoantigen-responsive gamma9delta2 T cells mediate protective tuberculosis immunity. J. Immunol. 181(7): 4471-4484
2. Voogt, P. J. et al. (1989)
  
  Normal hematopoietic progenitor cells and malignant lymphohematopoietic cells show different susceptibility to direct cell-mediated MHC-non-restricted lysis by T cell receptor
  ^–
  /CD3
  ^–
  , T cell receptor gamma delta
  ⁺
  /CD3
  ⁺
  and T cell receptor-alpha beta
  ⁺
  /CD3
  ⁺
  lymphocytes.
  
  J. Immunol. 142(5): 1774-1780
3. Borst, J. et al. (1988) Distinct molecular forms of human T cell receptor gamma/delta detected on viable T cells by a monoclonal antibody. J. Exp. Med. 167(5): 1625-1644
4. Lanier, L. L. et al. (1987)
  
  The T cell antigen receptor complex expressed on normal peripheral blood CD4
  ^–
  , CD8
  ^–
  T lymphocytes. A CD3-associated disulfide-linked gamma chain heterodimer.
  
  J. Exp. Med. 165(4): 1076-1094
5. Conrad, M. L. et al. (2007) TCR and CD3 antibody cross-reactivity in 44 species. Cytometry A 71(11): 925-933
6. Lanier, L. L. et al. (1988) Structural and serological heterogeneity of γ/δ T cell antigen receptor expression in thymus and peripheral blood. Eur. J. Immunol. 18(12): 1985-1992
7. Testi, R. and Lanier, L. L. (1989) Functional expression of CD28 on T cell antigen receptor gamma/delta-bearing T lymphocytes. Eur. J. Immunol. 19(1): 185-188
8. Lanier, L. L. et al. (1987) The gamma T-cell antigen receptor. J. Clin. Immunol. 7(6): 429-440
9. Moens, E. et al. (2011) IL-23R and TCR signaling drives the generation of neonatal Vγ9 Vδ2 T cells expressing high levels of cytotoxic mediators and producing IFN-γ and IL-17. J. Leukoc. Biol. 89(5): 743-752

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Anti-TCRg/d-Biotin, human, 11F2, 30 t

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Alternative names

Technical specifications

Figure 1

Selected references

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