Alternative names

Technical specifications

• Antigen: CD133/2
• Clone: REA820
• Isotype: recombinant human IgG1
• Isotype control: REA Control (S) antibodies
• Alternative names of antigen: PROM1, CORD12, MCDR2, MSTP061, PROML1, RP41, STGD4
• Entrez Gene ID: 8842
• Molecular mass of antigen [kDa]: 117
• Distribution of antigen: brain, endothelial cells, epithelial cells, heart, hematopoietic stem cells, kidney, liver, lung, pancreas, ES and iPS cells, red blood cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: PE, APC, PE-Vio615, PE-Vio770, VioBright 515, Biotin

• Figure 1

  Human peripheral blood mononuclear cells (PBMCs) were stained with CD133/2 antibodies or with the corresponding REA Control (S) antibodies (left image) as well as with CD34 antibodies. Flow cytometry was performed using the MACSQuant ® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

• Selected references

  1. Lai, J. et al. (2014)
     Id1 and NF-κB promote the generation of CD133

     ⁺

     and BMI-1

     ⁺

     keratinocytes and the growth of xenograft tumors in mice.
     Int. J. Oncol. 44(5): 1481-1489