YILIMART - Anti-PIR-A/B-Biotin, mouse 60 tests

Clone REA472 recognizes both isoforms of the mouse paired immunoglobulin-like receptor (PIR-A and PIR-B) antigen, a single-pass type I membrane protein which is also known as leukocyte immunoglobulin-like receptor 3 (LIR-3). Cell surface expression of PIR molecules on myeloid and B lineage cells increase with cellular differentiation and activation. PIR molecules are also expressed on dendritic cells, monocytes/macrophages, and mast cells in varying levels, but not on T cells and NK cells. PIR-A/B is a receptor for class I MHC antigens and down-regulates antigen-induced B cell–activation by recruiting phosphatases to its immunoreceptor tyrosine-based inhibitor motifs (ITIM).

Additional information: Clone REA472 displays negligible binding to Fc receptors.

Alternative names

6M21, Ly89, PIRA1, Pir, GP91, LIR-3, LILRB3, PIR-B

Technical specifications

Antigen: PIR-A/B
Clone: REA472
Isotype: recombinant human IgG1
Isotype control: REA Control antibodies
Alternative names of antigen: 6M21, Ly89, PIRA1, Pir, GP91, LIR-3, LILRB3, PIR-B
Entrez Gene ID: 18722, 18733
Molecular mass of antigen [kDa]: 83
Distribution of antigen: B cells, dendritic cells, macrophages, monocytes, mast cells
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: Biotin, VioBright FITC, PE, APC

Figure 1

Splenocytes from C57BL/6 mice were stained with Anti-PIR-A/B antibodies or with the corresponding REA Control antibodies (left image) as well as with CD45R (B220) antibodies. Flow cytometry was performed with the MACSQuant

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Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Selected references
1. Kubagawa, H. et al. (1997) A novel pair of immunoglobulin-like receptors expressed by B cells and myeloid cells. Proc. Natl. Acad. Sci. U.S.A. 94(10): 5261-5266
2. Bléry, M. et al. (1998) The paired Ig-like receptor PIR-B is an inhibitory receptor that recruits the protein-tyrosine phosphatase SHP-1. Proc. Natl. Acad. Sci. U.S.A. 95(5): 2446-2451
3. Kubagawa, H. et al. (1999) Biochemical nature and cellular distribution of the paired immunoglobulin-like receptors, PIR-A and PIR-B. J. Exp. Med. 189(2): 309-318

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Anti-PIR-A/B-Biotin, mouse 60 tests

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Alternative names

Technical specifications

Figure 1

Selected references

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