Alternative names

Technical specifications

• Antigen: MICA/MICB
• Clone: 6D4
• Isotype: mouse IgG2aκ
• Isotype control: Mouse IgG2a – isotype control antibodies
• Alternative names of antigen: MICB, PERB11.2, MICA, PERB11.1
• Distribution of antigen: endothelial cells, fibroblasts
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: VioBright FITC, PE, APC, PE-Vio770, APC-Vio770, Biotin

• Figure 1

  HeLa cells were stained with Anti-MICA/MICB antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. The specificity of the conjugated antibodies was confirmed by blocking the binding to the ligand, using pure unconjugated antibodies (left peak). Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide or DAPI fluorescence as in the case of tandem-conjugates.

• Selected references

  1. Li, P. et al. (1999) Crystal structure of the MHC class I homolog MIC-A, a γδ T cell ligand. Immunity 10: 577-584
  2. Houchins, J. P. et al. (1991) DNA sequence analysis of NKG2, a family of related cDNA clones encoding type II integral membrane proteins on human natural killer cells. J. Exp. Med. 173(4): 1017-1020
  3. Bauer, S. et al. (1999) Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA. Science 285: 727-729