YILIMART - Anti-IL-12 (p40/p70)-APC, human 30 tests

Interleukin 12 (IL-12) is a 70–75 kDa heterodimeric cytokine composed of a 40 kDa heavy chain and a 35 kDa light chain. It is produced mainly by monocytes and macrophages as well as dendritic cells, primarily in response to stimulation with bacterial or viral antigens. IL-12 has also been described to be produced by non-hematopoietic cells such as keratinocytes and epidermoid carcinomas. IL-12 displays various effects on both T and NK cells, playing an essential role in the interaction between innate and adaptive immunity, for example, it induces the production of IFN-γ alone or in synergy with IL-2, is a co-stimulator of both NK and T cell proliferation, and enhances cytolytic activity of both cell types. Moreover, IL-12 promotes the development of T helper 1 (Tʜ1)-specific immune responses by promoting the differentiation of CD4

⁺

cells towards a Tʜ1 phenotype while inhibiting the production of Tʜ2 cells.

Clone C8.6 reacts with both, the p40 chain and the p70 heterodimer of human, rhesus monkey, and cynomolgus monkey IL-12.

Alternative names

IL12B, CLMF, CLMF2, Il-12b, IMD28, IMD29, NKSF, NKSF2

Technical specifications

Antigen: IL-12 (p40/p70)
Clone: C8.6
Isotype: mouse IgG1κ
Isotype control: Mouse IgG1 – isotype control antibodies
Alternative names of antigen: IL12B, CLMF, CLMF2, Il-12b, IMD28, IMD29, NKSF, NKSF2
Entrez Gene ID: 3593
Molecular mass of antigen [kDa]: 35
Cross-reactivity:

rhesus monkey (
Macaca mulatta
)

,

cynomolgus monkey (
Macaca fascicularis
)
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: PE, APC

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left images) or stimulated with LPS / R848 for 6 hours. After 2 hours, brefeldin A was added. Cells were fixed, permeabilized, intracellularly stained with Anti-IL-12 (p40/p70) antibodies, and surface stained with CD14 antibodies. Cells were analyzed by flow cytometry.

Figure 2

Rhesus monkey PBMCs were either left unstimulated (left image) or stimulated with LPS/R848 for 6 hours. After 2 hours, brefeldin A was added. Cells were fixed, permeabilized, and intracellularly stained with Anti-IL-12 (p40/p70) antibodies. Cells were analyzed by flow cytometry.

Figure 3

Cynomolgus monkey PBMCs were either left unstimulated (left image) or stimulated with LPS / R848 for 6 hours. After 2 hours, brefeldin A was added. Cells were fixed, permeabilized, and intracellularly stained with Anti-IL-12 (p40/p70)-APC. Cells were analyzed by flow cytometry.

Selected references
1. Trinchieri, G. et al. (2003) Interleukin-12 and the regulation of innate resistance and adaptive immunity. Nat. Rev. Immunol. 3(2): 133-146
2. D´Andrea, A. et al. (1992) Production of natural killer cell stimulatory factor (interleukin 12) by peripheral blood mononuclear cells. J. Exp. Med. 176: 1387-1398

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Anti-IL-12 (p40/p70)-APC, human 30 tests

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Alternative names

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Selected references

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