Alternative names

Technical specifications

• Antigen: CD158b (KIR2DL2/DL3)
• Clone: DX27
• Isotype: mouse IgG2aκ
• Isotype control: Mouse IgG2a – isotype control antibodies
• Alternative names of antigen: KIR2DL2, KIR2DL3
• Entrez Gene ID: 3803
• Molecular mass of antigen [kDa]: 35-36
• Distribution of antigen: NK cells, T cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: PE, APC, PE-Vio770, pure

• Figure 1

  Human peripheral blood mononuclear cells (PBMCs) were stained with CD158b (KIR2DL2/DL3) antibodies as well as with CD56 antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

• Selected references

  1. Farag, S. S. et al. (2002) Natural killer cell receptors: new biology and insights into the graft-versus-leukaemia effect. Blood 100(6): 1935-1947
  2. Hsu, K. et al. (2002) The killer cell immunoglobin-like (KIR) genomic region: gene-order, haplotypes and allelic polymorphism. Immunol. Rev. 190: 40-52
  3. Selvakumar, A. et al. (1997) Polymorphism and domain variability of human killer cell inhibitory receptors. Immunol. Rev. 155: 183-196

• Customer reports and application notes

  Flow cytometry analysis of whole-blood NK cells expressing single killer cell immunoglobulin–like receptors