YILIMART - Adult NSC Sorting & Analysis Kit, mouse

Adult Neural Stem Cell Sorting and Analysis Kit, mouse enables a reliable identification of neural stem cells (NSCs) from the subventricular zone (SVZ) of mouse brain tissue for subsequent sorting and analysis. The kit contains a 3-color antibody cocktail, including two NSC-specific antibodies (anti-GLAST and anti-Plexin-B2) and three exclusion antibodies to exclude erythrocytes, leukocytes, microglia, neurons, ependymal cells, and neuroblasts. A workflow protocol is established for fast and pure isolation of NSCs (total process time 2h, purity >95%, viability >93%).

Applications

Isolation and analysis of adult neural stem cells (NSCs) in mouse subventricular zone (SVZ), including wild type mouse, transgenic mouse, or mouse disease models
Cultivation of NSCs for neurosphere assay and differentiation assay
Functional assays of isolated adult NSCs, such as gene overexpression and knockdown and transplantation, or pharmacological screening and molecular profiling

Figure 1

Dissociation of subventricular zone tissue yielded 3.69×10

⁵

± 9.3×10

⁴

cells per mouse (7–9 weeks old) with a viability rate of >97% (n = 12), using Neural Tissue Dissociation Kit (T) and gentleMACS™ Octo with Heaters.

This figure shows a representative analysis of the different fractions after exclusion of debris, doublets, and dead cells. Sorting of 3.69×10

⁵

± 9.3×10

⁴

total cells resulted in 36,000 ± 8,000 GLAST

⁺

Plexin-B2

⁺

neural stem cells with a purity of >95% and a viability rate of >93% as analyzed by propidium iodide staining (n = 6).

Non-sorted cells	Positive fraction	Negative fraction

Figure 2
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Figure 2

Cultivation of isolated NSCs under the specified assay conditions led to formation of a large number of neurospheres, which gave rise to secondary neurospheres (A, B).Neurospheres differentiated into glial cells as well as neurons as shown by expression of GFAP, Nestin, GLAST, MAP2, and O4 (C–F).

Cultivation of isolated NSCs under the specified assay conditions led to formation of a large number of neurospheres, which gave rise to secondary neurospheres (A, B).Neurospheres differentiated into glial cells as well as neurons as shown by expression of GFAP, Nestin, GLAST, MAP2, and O4 (C–F).