YILIMART - MSC Enummeration Kit, human

The MSC Enumeration Kit was developed for the standardized identification and quantification of human MSCs from fresh bone marrow aspirate by flow cytometry.

The MSC Enumeration Kit includes:

cocktail of three fluorochrome-conjugated antibodies including MSCs characterization marker CD271 (LNGFR) and exclusion markers CD45 and CD235a
additional MSC marker MSCA-1 (W8B2), for the identification of MSCs with highly proliferative potential
cocktail of four fluorochrome-conjugated isotype control antibodies
cell viability marker 7-AAD
Red Blood Cell Lysis Solution to remove erythrocytes
FcR Blocking Reagent to prevent unspecific binding

Background information

The success of bone marrow (BM)-derived mesenchymal stem cell (MSC) experiments depends on the quality of bone marrow aspirate (BMA), as well as the number of MSCs in the BMA sample. Commonly, MSC quantification is performed by CFU-F (colony-forming unit fibroblast) assay, which are time consuming and highly variable.

Based on the finding that the number of CFU-F colonies generated after 14 days of BM MNC (bone marrow mononuclear cell) culture is linearly related to the number of CD271

^bright

cells in BMA

¹

, we’ve developed a reliable procedure for absolute quantification of MSCs in BMA.

Selected references
1. Cuthbert, R. et al. (2012) Single-platform quality control assay to quantify multipotential stromal cells in bone marrow aspirates prior to bulk manufacture or direct therapeutic use. Cytotherapy 14(4): 431-440
Brochures and posters

MSC Enumeration Kit

Workflow for human mesenchymal stem or stromal cells
Scientific posters

Standardized quantification of human bone marrow mesenchymal stromal cells based on a flow cytometry assay - ISCT 2015

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MSC Enummeration Kit, human

For research use only

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Background information

Selected references

Brochures and posters

Scientific posters

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