Background information

• Figure 1

  Example of immunofluorescent staining with the Treg Detection Kit (CD4/CD25/CD127) using whole blood and the lyse/no wash protocol with CD45 trigger. A–E depict the gating strategy for the flow cytometric analysis of CD4 + CD25 high CD127 dim/ – cells.

  A:	B:
  An appropriate threshold was defined based on CD45-VioBlue versus side scatter signals, for the exclusion of debris and erythrocytes from the data.	Lymphocytes were gated for their expected morphology using a forward scatter versus side scatter "lymphocyte gate" (P1)
  C:	D:
  Dead cells were excluded by a CD127-PE versus propidium iodide dot plot (P2).	CD4 + lymphocytes were gated in P3.
  CD25 high CD127 dim/– Treg cells were gated by a CD127-PE versus CD25-APC dot plot.

• Selected references

  1. Seddiki, N. et al. (2006) Expression of interleukin (IL)-2 and IL-7 receptors discriminates between human regulatory and activated T cells. J. Exp. Med. 203: 1693-1700
  2. Liu, W. et al. (2006)
     CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4

     ⁺

     T reg cells.
     J. Exp. Med. 203: 1701-1711

• Brochures and posters

  Regulatory T cell research