YILIMART - IFN-a Secretion Assay Det Kit (PE)

The IFN-α Secretion Assay was developed for the sensitive detection of human IFN-α–secreting cells.

Background information

Type I interferons (IFNs), including IFN-α, are a family of cytokines that have pleiotropic functions in the immune system.

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Besides its potent antiviral activity, IFN-α exerts a variety of immunomodulatory effects, including positive and negative regulation of its own production, regulation of IL-12 and IFN-γ production, promotion of natural killer (NK) cell cytotoxicity, activation and maturation of dendritic cells, and the promotion of B cell development towards plasma cells.

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IFN-α is expressed predominantly by CD123

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CD11c

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CD303 (BDCA2)

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plasmacytoid dendritic cells, also referred to as natural IFN-producing cells. These cells representing approximately 0.4% of the total PBMCs, produce up to 1000-fold more type I IFNs than any other cell type. However, biologically relevant levels of IFN-α are also produced by other cell types, e.g., monocytes and myeloid dendritic cells.

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Downstream applications

The IFN-α Secretion Assay can be used for the detection of viable IFN-α–secreting leukocytes or the detection of IFN-α–secreting cells for enumeration and phenotypic analysis. Furthermore, the stimulatory or regulatory functions of cytokine-secreting monocytes and dendritic cells can be analysed.

Figure 1

Human PBMCs were incubated with or without CpG ODN 2216 for 5 hours. Cells were stained using the IFN-α Secretion Assay – Detection Kit (PE) and CD303 (BDCA-2)-APC and analyzed by flow cytometry.

Stimulated sample	Unstimulated control

Selected references
1. Bangham, C. R. M. et al. (2012) Systems biology approaches reveal a specific interferon-inducible signature in HTLV-1 associated myelopathy. PLoS Pathog. 8(1)
2. Biron, C. A. (2001) Immunity 14: 661-664
3. Montoya, M. et al. (2002) Type I interferons produced by dendritic cells promote their phenotypic and functional activation. Blood 99: 3263-3271
4. Santini, S. M. et al. (2002) The natural alliance between type I interferon and dendritic cells and its role in linking innate and adaptive immunity. Proc. Natl. Acad. Sci. U.S.A. 22: 1071-1080
5. Theofilopoulos, A. N. et al. (2005) Type I interferons (alpha/beta) in immunity and autoimmunity. Annu. Rev. Immunol. 23: 307-336

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IFN-a Secretion Assay Det Kit (PE)

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Background information

Downstream applications

Figure 1

Selected references

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