Downstream applications

• Figure 1

  Anti-Biotin MACSiBead Particles were loaded with CD2, CD3, and CD28 antibodies. T cells were isolated using the Pan T Cell Isolation Kit. Purified T cells were activated for 72 hours using one loaded Anti-Biotin MACSiBead Particle per two T cells. The negative control experiment was performed without adding MACSiBead Particles. Cells were fluorescently stained using CD4-FITC and CD25-PE or CD4-PE and CD69-FITC.

  Stimulated sample	Unstimulated control
  Stimulated sample	Unstimulated control

• Selected references

  1. Lécureuil et al. (2007) Trapping and apoptosis of novel subsets of memory T lymphocytes expressing CCR6 in the spleen of HIV-infected patients. Blood 109: 3649-3657
  2. Acosta-Rodriguez, E. V. et al. (2007) Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells. Nat. Immunol. 8: 942-948
  3. Sattler et al. (2009) Cytokine-induced human IFN-gamma-secreting effector-memory Th cells in chronic autoimmune inflammation. Blood 113: 1948-1956
  4. Diveu, C. et al. (2009) IL-27 blocks RORc expression to inhibit lineage commitment of Th17 cells. J. Immunol. 182: 5748-5756
  5. Camargo, J. F. et al. (2009) CCR5 expression levels influence NFAT translocation, IL-2 production, and subsequent signaling events during T lymphocyte activation. J. Immunol. 182: 171-182
  6. Duraisingham, S. S. et al. (2009) TLR-stimulated CD34 stem cell-derived human skin-like and monocyte-derived dendritic cells fail to induce Tʜ17 polarization of naive T cells but do stimulate Tʜ1 and Tʜ17 memory responses. J. Immunol. 183: 2242-2251
  7. Wieckowski, E. U. et al. (2009)
     Tumor-derived microvesicles promote regulatory T cell expansion and induce apoptosis in tumor-reactive activated CD8

     ⁺

     T lymphocytes.
     J. Immunol. 183: 3720-3730