Background information

• Figure 1

  View details

  Figure 1

  CD4

  ⁺

  CD25

  ⁺

  regulatory T cells, isolated with the CD4

  ⁺

  CD25

  ⁺

  Regulatory T Cell Isolation Kit, were cocultured with CD4

  ⁺

  CD25

  ^-

  responder T cells in different ratios. For T cell stimulation, the Treg Suppression Inspector was added to the culture. As controls, CD4

  ⁺

  CD25

  ⁺

  Treg cells and CD4

  ⁺

  CD25

  ^-

  responder T cells alone were cultured without any stimulus. Proliferation of T cells was determined by measuring

  ³

  H-thymidine incorporation.

  
  CD4

  ⁺

  CD25

  ⁺

  regulatory T cells, isolated with the CD4

  ⁺

  CD25

  ⁺

  Regulatory T Cell Isolation Kit, were cocultured with CD4

  ⁺

  CD25

  ^-

  responder T cells in different ratios. For T cell stimulation, the Treg Suppression Inspector was added to the culture. As controls, CD4

  ⁺

  CD25

  ⁺

  Treg cells and CD4

  ⁺

  CD25

  ^-

  responder T cells alone were cultured without any stimulus. Proliferation of T cells was determined by measuring

  ³

  H-thymidine incorporation.

• Selected references

  1. Kleinewietfeld et al. (2009) CD49d provides access to "untouched" human Foxp3+ Treg free of contaminating effector cells. Blood 113: 827-836
  2. Mougiakakos, D. et al. (2009) Naturally occurring regulatory T cells show reduced sensitivity toward oxidative stress-induced cell death. Blood 113: 3542-3545

• Brochures and posters

  Regulatory T cell research

• Customer reports and application notes

  Human CD4

  ⁺

  CD25

  ⁺

  regulatory T cell isolation,

  in vitro

  suppression assay and analysis - Workflow protocol