YILIMART - Blood DC Enumeration Kit,human-100 tests

The Blood Dendritic Cell Enumeration Kit was developed for easy identification and enumeration of dendritic cells (DCs) and DC subsets in whole blood or PBMCs by flow cytometry. All reagents required for the analysis are included in the kit.

Applications of the kit include monitoring of peripheral blood DC frequency and number, for example, after hematopoietic stem cell mobilization

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, or in the course of pathogenic infections, for example by HIV.

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Furthermore, DC numbers were determined in patients with chronic graft versus host disease

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, inflammatory bowel disease

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, psoriatic arthritis and rheumatoid arthritis

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, under long-term immunosuppression

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, lymphoproliferative disease of granular lymphocytes

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, or in patients with hepatocellular carcinoma, in order to analyze the immunosuppressive role of IL-10 on circulating dendritic cells

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.

Background information

The kit is based on the dendritic cell-specific surface antigens CD303 (BDCA-2), CD141 (BDCA-3), and CD1c (BDCA-1).

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The markers allow easy identification of three distinct dendritic cell subsets in blood: plasmacytoid dendritic cells (PDCs) are identified by CD303 (BDCA-2), type-1 myeloid dendritic cells (MDC1s) are identified by CD1c (BDCA-1), and type-2 myeloid dendritic cells (MDC2s) are identified by CD141 (BDCA-3) expression. The distinct dendritic cell subsets are analyzed within one sample by three-color (PDCs and MDC1s) or four-color (PDCs, MDC1s and MDC2s) flow cytometry.

The kit includes all reagents needed for a complete analysis: pre-mixed antibody cocktails for dendritic cell detection and for isotype control, a dead cell discrimination dye, and ready-to-use reagents for erythrocyte lysis and cell fixation. The standardized reagents and protocol ensure reproducible results.

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The dead cell dye used in this kit allows staining of dead cells in combination with cell fixation. This is particularly useful when working with hazardous samples.

The assay requires only small samples (either 300 μL of whole blood or 1×10

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PBMCs, both for sample and control).

Figure 1
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Figure 1

Dendritic cell subset–specific antigens.

Dendritic cell subset–specific antigens.
Figure 2
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Figure 2

Staining procedure of the Blood Dendritic Cell Enumeration Kit.

Staining procedure of the Blood Dendritic Cell Enumeration Kit.

Selected references
1. Dzionek, A. et al. (2000) BDCA-2, BDCA-3, BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood. J. Immunol. 165: 6037-6046
2. Dzionek, A. et al. (2002) Plasmacytoid dendritic cells: from specific surface markers to specific cellular functions. Hum. Immunol. 36: 1133-1148
3. Arpinati, M. et al. (2002) Use of anti-BDCA-2 antibody for detection of dendritic cell type-2 (DC2) in allogeneic hematopoietic stem cell transplantation. Bone Marrow Transplant. 29: 887-891
4. Chehimi, J. et al. (2002) Persistent decreases in blood plasmacytoid dendritic cell number and function despite effective highly active antiretroviral therapy and increased blood myeloid dendritic cells in HIV-infected individuals. J. Immunol. 168: 4769-4801
5. Clark, F. J. et al. (2003) Origin and subset distribution of peripheral blood dendritic cells in patients with chronic graft-versus-host disease. Transplantation 75: 221-225
6. Baumgart, D. C. et al. (2005) Patients with active inflammatory bowel disease lack immature peripheral blood plasmacytoid and myeloid dendritic cells. Gut 54: 228-236
7. Hackstein, H. et al. (2005) Dendritic cell deficiency in the blood of kidney transplant patients on long-term immunosuppression: results of a prospective matched-cohort study. Am. J. Transplant. 5: 2945-2953
8. Zambello et al. (2005) Phenotypic and functional analyses of dendritic cells in patients with lymphoproliferative disease of granular lymphocytes (LDGL). Blood 106: 3926-3931
9. Jongbloed, S. L. et al. (2006) Enumeration and phenotypical analysis of distinct dendritic cell subsets in psoriatic arthritis and rheumatoid arthritis. Arthritis Res. Ther. 8: R15
Scientific posters

Blood DC preparations generated using automated CliniMACS Prodigy
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CD1c/CD304 enrichment and activation system efficiently activate CD8
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antigen-specific T cells. 14th CIMT Annual Meeting (2016)

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Blood DC Enumeration Kit,human-100 tests

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Background information

Figure 1

Figure 1

Figure 2

Figure 2

Selected references

Scientific posters

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