YILIMART - Melanoma Cell Enrichm.&Det.Kit, h

The Melanoma Cell Enrichment and Detection Kit was developed for the enrichment of melanoma cells with Anti-Melanoma (MCSP) MicroBeads and subsequent immunocytochemical detection with the melanoma-specific Anti-Melanoma (MCSP)-Alkaline Phosphatase from blood, bone marrow, and lymphoid tissue of patients with stage I–IV melanoma

¹

.

Background information

The Anti-Melanoma (MCSP) MicroBeads, clone 9.2.27, and the Anti-Melanoma (MCSP)-Alkaline Phosphatase, clone EP1, bind to different epitopes of the melanoma-associated chondroitin sulfate proteoglycan (MCSP). MCSP is also known as high molecular weight melanoma-associated antigen, or NG2, which is expressed on melanoma cells but not on carcinoma cells, fibroblastoid cells, or cells of hematopoietic origin.

Detailed procedure

Cells are labeled with Anti-Melanoma MicroBeads and positively selected on a first separation column. Then, melanoma cells are immobilized within a second MACS® Column and stained immunocytochemically with Anti-Melanoma (MCSP)-Alkaline Phosphatase. During the entire staining procedure, the cells remain within the magnetic field of the column. Thus, cell loss is minimized. After elution from the column, cells are fixed, transferred onto a microscope slide, incubated with the substrate, and are then ready for analysis by light microscopy.

Columns

For enrichment and in-column staining: MS Columns.

Figure 1
View details

Figure 1

Cells of the melanoma cell line SK-MEL-28 were spiked into 5×10
⁷
PBMCs of a healthy donor. Thereafter, the cells were enriched and analyzed using the Melanoma Cell Enrichment and Detection Kit.

Cells of the melanoma cell line SK-MEL-28 were spiked into 5×10
⁷
PBMCs of a healthy donor. Thereafter, the cells were enriched and analyzed using the Melanoma Cell Enrichment and Detection Kit.

Brochures and posters

Cancer Research

查看数据文件查看下载

Melanoma Cell Enrichm.&Det.Kit, h

For research use only

您浏览过的商品

Background information

Detailed procedure

Columns

Figure 1

Figure 1

Brochures and posters

相关产品