YILIMART - Human IL-13, premium grade (10 μg)

IL-13 stands for interleukin 13. Human IL-13 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human IL-13 can be used for a variety of applications, including:

Inhibition of monocyte and macrophage activation.
B cell activation.
NK cell and LAK cell stimulation.

Background information

Interleukin 13 (IL-13) is an immunoregulatory protein, which shares similarity in structure and function with IL-4. IL-13 is mainly secreted by Tʜ2 cells, as well as mast cells and NK cells, and binds a multi-subunit receptor, including the alpha chain of the IL-4 receptor, which downstream activates the signal transducer and activator of transcription 6 (STAT-6). Although IL-13 is involved in the pathogenesis of airway diseases such as asthma, it also plays a protective role for the mucosa, via induction of matrix metalloproteinases (MMPs) and other anti-inflammatory effects. Similarly to IL-4, IL-13 induces humoral responses favoring B cell proliferation and isotype switching to IgE, whereas, on monocytes and macrophages, it counteracts the effects of IL-1β, TNF-α, IL-6, by inhibiting the secretion of inflammatory cytokines and increasing IL-1RA expression. IL-13 acts on hematopoietic differentiation and can synergize with IL-2 in the activation of IFN-γ mediated killer activity of lymphokine activated killer (LAK) cells. The protein sequences of human and mouse IL-13 share 58% identity and are cross species reactive.

Quality description

Research-grade

cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Premium-grade

cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

Proliferation of TF-1 cells (NIBSC 94/622)
premium grade: ≥ 4×

10
⁵
U/mg
research grade: ≥ 2×

10
⁵
U/mg

Figure 1
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Figure 1

Mass spectrometry analysis (ESI-MS) of Human IL-13, premium grade. The peak corresponds to the calculated molecular mass of 12524 Da.

Mass spectrometry analysis (ESI-MS) of Human IL-13, premium grade. The peak corresponds to the calculated molecular mass of 12524 Da.
Figure 2
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Figure 2

SDS-PAGE of Human IL-13, premium grade
under reduced (R) and non-reduced (NR) conditions.

SDS-PAGE of Human IL-13, premium grade
under reduced (R) and non-reduced (NR) conditions.
Figure 3
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Figure 3

Human IL-13 biological activity.
Activity of Human IL-13, premium grade (red bar) was compared to another commercially available product (black bar).

Human IL-13 biological activity.
Activity of Human IL-13, premium grade (red bar) was compared to another commercially available product (black bar).
Figure 4
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Figure 4

Human IL-13 activity assay.
The biological activity of Human IL-13, premium grade was determined by proliferation assay using TF-1 cells.

Human IL-13 activity assay.
The biological activity of Human IL-13, premium grade was determined by proliferation assay using TF-1 cells.

Selected references
1. Diedrich, C. R. et al. (2013) Monocyte-derived IL-5 reduces TNF production by Mycobacterium tuberculosis-specific CD4 T cells during SIV/M. tuberculosis coinfection. J. Immunol. 190(12): 6320-6328
2. Kitamura, T. et al. (1989) Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J. Cell. Physiol. 140: 323-334
3. Borriello, F. et al. (2015) IL-3 synergizes with basophil-derived IL-4 and IL-13 to promote the alternative activation of human monocytes. Eur. J. Immunol. 45(7): 2042-2051
Certificates

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Human IL-13, premium grade (10 μg)

For research use only

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Applications

Background information

Quality description

Biological activity

Figure 1

Figure 1

Figure 2

Figure 2

Figure 3

Figure 3

Figure 4

Figure 4

Selected references

Certificates

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