Background information
CDC (complement dependent cytotoxicity) research requires the isolation of lymphocyte subpopulations. Enrichment of cells for these assays can be exceedingly time consuming or result in co-enrichment of unwanted or non-viable cells. The MACSprep™ HLA Cell Isolation Kits allows the fast and easy isolation of B and T lymphocytes directly from samples without density gradient centrifugation or erythrocyte lysis.
Detailed separation procedure
The MACSprep™ HLA Cell Isolation Kits allow the untouched isolation of human lymphocytes from anticoagulated whole blood or spleen cell suspensions. Non-target cells are removed by immunomagnetic depletion while erythrocytes are sedimented, yielding viable untouched target cells of high purity.
The MACSprep HLA Starting Kit MACSxpress contains all necessary components to process up to 25mL per sample,
two samples in parallel. The MACSprep HLA Starting Kit MACSiMAG contain all necessary components to process up to 16mL per sample,
three to eight samples in parallel. Downstream applications
Isolated cells are immediately ready for downstream HLA research.
