YILIMART - Human BMP-4, premium grade (1000 μg)

Recombinant human BMP-4 can be used for the differentiation of human embryonic and induced pluripotent stem cells, as in into cells of the endodermal and mesodermal lineage or into hematopoietic stem cells and progenitors. The bone morphogenetic protein (BMP)-4 belongs to the TGF-β superfamily and has diverse pivotal roles during bone and cartilage formation, tissue repair, or organ and neuronal development. BMP-4 recombinant protein can ideally be used for functional assays, cell culture, and differentiation studies.

Applications

Human BMP-4 can be used for a variety of applications, including:

Differentiation of human ES and iPS cells.
Maintenance of mouse ESCs under serum-free conditions.

Background information

Bone morphogenetic protein 4 (BMP-4) is a member of the bone morphogenetic protein family, which is part of the TGF-β superfamily. BMP-4 plays an important role in early differentiation of the embryo and in establishing of a dorsal-ventral axis. It is involved in bone and cartilage development and has been shown to play a role in the differentiation of sympathetic neurons. In synergy with Activin A it promotes the formation of definitive endoderm in human embryonic stem cells (ESCs). Furthermore, BMP-4 supports LIF (leukaemia inhibitory factor) as a positive factor for mouse ESC self-renewal and can replace the requirement of serum. The amino acid sequences of mature human BMP-4 shares 98% identity with mouse or rat BMP-4.

Quality description

Research-grade

cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Biological activity

Induction of alkaline phosphatase assay using mouse C2C12 cells
premium grade: ≥ 8×

10
⁴
U/mg
research grade: ≥ 4×

10
⁴
U/mg

Figure 1
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Figure 1

SDS-PAGE of Human BMP-4, premium grade
under reduced (R) and non-reduced (NR) conditions.

SDS-PAGE of Human BMP-4, premium grade
under reduced (R) and non-reduced (NR) conditions.
Figure 2
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Figure 2

Human BMP-4 activity assay.
The biological activity of Human BMP-4, premium grade was determined by alkaline phosphatase assay using mouse C2C12 fibroblasts.

Human BMP-4 activity assay.
The biological activity of Human BMP-4, premium grade was determined by alkaline phosphatase assay using mouse C2C12 fibroblasts.
Figure 3
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Figure 3

Human BMP-4 biological activity.
Activity of Human BMP-4, premium grade (red bar) was compared to another commercially available product (black bar).

Human BMP-4 biological activity.
Activity of Human BMP-4, premium grade (red bar) was compared to another commercially available product (black bar).

Selected references
1. Cheng, H. et al. (2003) Osteogenic activity of the fourteen types of human bone morphogenetic proteins (BMPs). J Bone Joint Surg Am 85(A8): 1544-1552
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Human BMP-4, premium grade (1000 μg)

For research use only

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Applications

Background information

Quality description

Biological activity

Figure 1

Figure 1

Figure 2

Figure 2

Figure 3

Figure 3

Selected references

Certificates

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