YILIMART - Mouse IL-1b, premium gr. (1000 μg)

IL-1β stands for interleukin 1β, also termed catabolin or IFN-β inducing factor. Mouse IL-1β is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Mouse IL-1β can be used for for a variety of applications including:

Activation of antigen-presenting cells
Polarization of T_H 17 cells
Investigation of IL-1 receptor signaling

Alternative names

IL-1F2, Catabolin, MCF

Background information

Interleukin 1β (IL-1β) is a proinflammatory cytokine secreted mainly by monocytes and macrophages. IL-1β secretion has also been reported for a variety of other cells, including B cells, NK cells, dendritic cells, astrocytes, and microglial cells. It mediates inflammatory responses in B cells, T cells, and NK cells by inducing the production of cytokines, such as IL-2, IL-3, IL-6, as well as interferons. Upon exposure to IL-1β, endothelial cells and smooth muscle cells synthesize prostaglandins and other derivatives of arachidonic acid. In addition, during arthritic diseases, IL-1β causes degranulation of basophils and eosinophils as well as activation of osteoclasts. IL-1β is mitogenic for mesangial cells, glial cells, and keratinocytes.

Quality description

Research-grade

cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Premium-grade

cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

Proliferation of D10.G4.1 cells (NIBSC 93/668)
premium grade: ≥ 8×

10
⁸
U/mg
research grade: ≥ 5×

10
⁸
U/mg

Figure 1
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Figure 1

Mass spectrometry analysis (ESI-MS) of Mouse IL-1ß, premium grade. The peak corresponds to the calculated molecular mass of 17394 Da.

Mass spectrometry analysis (ESI-MS) of Mouse IL-1ß, premium grade. The peak corresponds to the calculated molecular mass of 17394 Da.
Figure 2
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Figure 2

Mouse IL-1β biological activity.
Activity of Mouse IL-1β, premium grade, (red bar) was compared to another commercially available product (black bar).

Mouse IL-1β biological activity.
Activity of Mouse IL-1β, premium grade, (red bar) was compared to another commercially available product (black bar).
Figure 3
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Figure 3

SDS -PAGE of Mouse IL-1β, premium grade
under reducing (R) and non-reducing (NR) conditions.

SDS -PAGE of Mouse IL-1β, premium grade
under reducing (R) and non-reducing (NR) conditions.
Figure 4
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Figure 4

Mouse IL-1β activity assay.
The biological activity of Mouse IL-1β, premium grade was determined by proliferation assay using D10.G4.1 cells.

Mouse IL-1β activity assay.
The biological activity of Mouse IL-1β, premium grade was determined by proliferation assay using D10.G4.1 cells.

Selected references
1. Vegran, F. et al. (2014) The transcription factor IRF1 dictates the IL-21-dependent anticancer functions of Tʜ9 cells. Nat. Immunol. 15(8): 758-766
Certificates

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Mouse IL-1b, premium gr. (1000 μg)

For research use only

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Applications

Alternative names

Background information

Quality description

Biological activity

Figure 1

Figure 1

Figure 2

Figure 2

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Figure 3

Figure 4

Figure 4

Selected references

Certificates

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